LOCUS       sequence-85346-e        1917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mApple-VASP-5
FEATURES             Location/Qualifiers
     source          1..1917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggt ctacattaag cacccagccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      361 attcacgtta accaggactc ctccctgcag gacggcgtgt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctccgagg agcggatgta ccccgaggac ggcgccctga agagcgagat caagaagagg
      541 ctgaagctga aggacggcgg ccactacgcc gccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cgtgtcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctatga gcgagacggt catctgttcc
      781 agccgggcta ctgtgatgct ttatgatgac agcaacaagc gatggctccc tgctggcact
      841 ggtccccagg ccttcagccg cgtccagata taccacaacc ccactgctaa ctccttccga
      901 gttgttggcc gcaagatgca gccggatcag caggtggtta tcaactgtgc catcattcgg
      961 ggtgtcaagt acaatcaggc cactcccatc ttccatcagt ggcgagatgc ccgccaggtc
     1021 tggggcctca acttcggcag caaggaggac gccatacagt ttgcaacagg catggccaac
     1081 gccctagagg ccttggaagg aggtgggcct cccccagccc cagcaccccc tgcctggtct
     1141 gcccagaatg gtccctcccc agaggagctg gaacaacaga aaaggcagcc ggagcatatg
     1201 gagcgccggg tctccaatgc aggaggccca cctgctcccc cagctggggg ccctcctcca
     1261 cctccaggac ctccccctcc tccaggtccc cccccacccc caggtctgcc ctcctcaggg
     1321 gtatctgggg caggtcatgg agcaggggca gccccacccc ctgcaccccc actccctaca
     1381 acacagggcc ccaatagtgg gggttccggg gccccaggcc tggctgctgc cattgctgga
     1441 gccaaactca ggaaagtgag caagcaggag gaggcctctg gggggcccct ggcccccaaa
     1501 gctgagaaca gtcgaagcac tggtgggggg cttatggaag agatgaacgc catgctggcc
     1561 cggagaagaa aagccacaca ggttggggag aagcccccca aagacgagtc agccagtgag
     1621 gagtcagagg cccgactccc tgcccagagt gaacctgtga gaagaccctg ggagaagaac
     1681 agcacaacct tgccaaggat gaagtcgtcc tcttctgtga ctacctccga ggcccacccc
     1741 tcgacgccct gctccagtga tgactccgac ttggagaggg tgaagcagga gcttctggaa
     1801 gaggtgcgga aggagctaca gaaaatgaaa gaggaaatca tcgaagtctt tgtccaggag
     1861 ctgaggaagc ggggttctcc ttgagcggcc gcgactctag atcataatca gccatac
//
