LOCUS       sequence-85348-e        2201 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 568, Emission: 
            592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2201)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2201
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1471..1480
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1477..2187
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1890..1909
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcccatg
       61 tccaccaggt ccgtgtcctc gtcctcctac cgcaggatgt tcggcggccc gggcaccgcg
      121 agccggccga gctccagccg gagctacgtg actacgtcca cccgcaccta cagcctgggc
      181 agcgcgctgc gccccagcac cagccgcagc ctctacgcct cgtccccggg cggcgtgtat
      241 gccacgcgct cctctgccgt gcgcctgcgg agcagcgtgc ccggggtgcg gctcctgcag
      301 gactcggtgg acttctcgct ggccgacgcc atcaacaccg agttcaagaa cacccgcacc
      361 aacgagaagg tggagctgca ggagctgaat gaccgcttcg ccaactacat cgacaaggtg
      421 cgcttcctgg agcagcagaa taagatcctg ctggccgagc tcgagcagct caagggccaa
      481 ggcaagtcgc gcctggggga cctctacgag gaggagatgc gggagctgcg ccggcaggtg
      541 gaccagctaa ccaacgacaa agcccgcgtc gaggtggagc gcgacaacct ggccgaggac
      601 atcatgcgcc tccgggagaa attgcaggag gagatgcttc agagagagga agccgaaaac
      661 accctgcaat ctttcagaca ggatgttgac aatgcgtctc tggcacgtct tgaccttgaa
      721 cgcaaagtgg aatctttgca agaagagatt gcctttttga agaaactcca cgaagaggaa
      781 atccaggagc tgcaggctca gattcaggaa cagcatgtcc aaatcgatgt ggatgtttcc
      841 aagcctgacc tcacggctgc cctgcgtgac gtacgtcagc aatatgaaag tgtggctgcc
      901 aagaacctgc aggaggcaga agaatggtac aaatccaagt ttgctgacct ctctgaggct
      961 gccaaccgga acaatgacgc cctgcgccag gcaaagcagg agtccactga gtaccggaga
     1021 caggtgcagt ccctcacctg tgaagtggat gcccttaaag gaaccaatga gtccctggaa
     1081 cgccagatgc gtgaaatgga agagaacttt gccgttgaag ctgctaacta ccaagacact
     1141 attggccgcc tgcaggatga gattcagaat atgaaggagg aaatggctcg tcaccttcgt
     1201 gaataccaag acctgctcaa tgttaagatg gcccttgaca ttgagattgc cacctacagg
     1261 aagctgctgg aaggcgagga gagcaggatt tctctgcctc ttccaaactt ttcctccctg
     1321 aacctgaggg aaactaatct ggattcactc cctctggttg atacccactc aaaaaggaca
     1381 cttctgatta agacggttga aactagagat ggacaggtta tcaacgaaac ttctcagcat
     1441 cacgatgacc ttgaagggga tccaccggtc gccaccatgg tgagcaaggg cgaggagaat
     1501 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
     1561 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggc ctttcagacc
     1621 gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
     1681 cagttcatgt acggctccaa ggtctacatt aagcacccag ccgacatccc cgactacttc
     1741 aagctgtcct tccccgaggg cttcaggtgg gagcgcgtga tgaacttcga ggacggcggc
     1801 attattcacg ttaaccagga ctcctccctg caggacggcg tgttcatcta caaggtgaag
     1861 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     1921 gaggcctccg aggagcggat gtaccccgag gacggcgccc tgaagagcga gatcaagaag
     1981 aggctgaagc tgaaggacgg cggccactac gccgccgagg tcaagaccac ctacaaggcc
     2041 aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga catcgtgtcc
     2101 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     2161 ggcggcatgg acgagctgta caagtaagcg gccgcgactc t
//
