LOCUS       sequence-85349-e        4039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Thievessen I, Thompson PM, Berlemont S, Plevock KM, Plotnikov SV, 
            Zemljic-Harpf A, Ross RS, Davidson MW, Danuser G, Campbell SL, 
            Waterman CM
  TITLE     Vinculin-actin interaction couples actin retrograde flow to focal 
            adhesions, but is dispensable for focal adhesion growth.
  JOURNAL   J Cell Biol. 2013 Jul 8;202(1):163-77. doi: 10.1083/jcb.201303129.
  PUBMED    23836933
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1083/jcb"; 
            journalName: "J Cell Biol"; date: "2013-07-8- 8"; volume: "202"; 
            issue: "1"; pages: "163-77"
COMMENT     Sequence Label: mApple-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             26..733
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     439..458
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccat
       61 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      121 cgagatcgag ggcgagggcg agggccgccc ctacgaggcc tttcagaccg ctaagctgaa
      181 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
      241 cggctccaag gtctacatta agcacccagc cgacatcccc gactacttca agctgtcctt
      301 ccccgagggc ttcaggtggg agcgcgtgat gaacttcgag gacggcggca ttattcacgt
      361 taaccaggac tcctccctgc aggacggcgt gttcatctac aaggtgaagc tgcgcggcac
      421 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccga
      481 ggagcggatg taccccgagg acggcgccct gaagagcgag atcaagaaga ggctgaagct
      541 gaaggacggc ggccactacg ccgccgaggt caagaccacc tacaaggcca agaagcccgt
      601 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcgtgtccc acaacgagga
      661 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      721 cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattcgg gaaactcgag
      781 aacaaagctt gaaaacggcc gcatgccagt gtttcatacg cgcacgatcg agagcatcct
      841 ggagccggtg gcacagcaga tctcccacct ggtgataatg cacgaggagg gcgaggtgga
      901 cggcaaagcc attcctgacc tcaccgcgcc cgtggccgcc gtgcaggcgg ccgtcagcaa
      961 cctcgtccgg gttggaaaag agactgttca aaccactgag gatcagattt tgaagagaga
     1021 tatgccacca gcatttatta aggttgagaa tgcttgcacc aagcttgtcc aggcagctca
     1081 gatgcttcag tcagaccctt actcagtgcc tgctcgagat tatctaattg atgggtcaag
     1141 gggcatcctc tctggaacat cagacctgct ccttaccttc gatgaggctg aggtccgtaa
     1201 aattattaga gtttgcaaag gaattttgga atatcttaca gtggcagagg tggtggagac
     1261 tatggaagat ttggtcactt acacaaagaa tcttgggcca ggaatgacta agatggccaa
     1321 gatgattgac gagagacagc aggagctcac tcaccaggag caccgagtga tgttggtgaa
     1381 ctcgatgaac accgtgaaag agttgctgcc agttctcatt tcagctatga agatttttgt
     1441 aacaactaaa aactcaaaaa accaaggcat agaggaagct ttaaaaaatc gcaattttac
     1501 tgtagaaaaa atgagtgctg aaattaatga gataattcgt gtgttacaac tcacctcttg
     1561 ggatgaagat gcctgggcca gcaaggacac tgaagccatg aagagagcat tggcctccat
     1621 agactccaaa ctgaaccagg ccaaaggttg gctccgtgac cctagtgcct ccccagggga
     1681 tgctggtgag caggccatca gacagatctt agatgaagct ggaaaagttg gtgaactctg
     1741 tgcaggcaaa gaacgcaggg agattctggg aacttgcaaa atgctagggc agatgactga
     1801 tcaagtggct gacctccgtg ccagaggaca aggatcctca ccggtggcca tgcagaaagc
     1861 tcagcaggta tctcagggtc tggatgtgct cacagcaaaa gtggaaaatg cagctcgcaa
     1921 gctggaagcc atgaccaact caaagcagag cattgcaaag aagatcgatg ctgctcagaa
     1981 ctggcttgca gatccaaatg gtggaccgga aggagaagag cagattcgag gtgctttggc
     2041 tgaagctcgg aaaatagcag aattatgtga tgatcctaaa gaaagagatg acattctacg
     2101 ttcccttggg gaaatatctg ctctgacttc taaattagca gatctacgaa gacaggggaa
     2161 aggagattct ccagaggctc gagccttggc caaacaggtg gccacggccc tgcagaacct
     2221 gcagaccaaa accaaccggg ctgtggccaa cagcagaccg gccaaagcag ctgtacacct
     2281 tgagggcaag attgagcaag cacagcggtg gattgataat cccacagtgg atgaccgtgg
     2341 agtcggtcag gctgccatcc gggggcttgt ggccgaaggg catcgtctgg ctaatgttat
     2401 gatggggcct tatcggcaag atcttctcgc caagtgtgac cgagtggacc agctgacagc
     2461 ccagctggct gacctggctg ccagagggga aggggagagt cctcaggcac gagcacttgc
     2521 atctcagctc caagactcct taaaggatct aaaagctcgg atgcaggagg ccatgactca
     2581 ggaagtgtca gatgttttca gcgataccac aactcccatc aagctgttgg cagtggcagc
     2641 cacggcgcct cctgatgcgc ctaacaggga agaggtattt gatgagaggg cagctaactt
     2701 tgaaaaccat tcaggaaagc ttggtgctac ggccgagaag gcggctgcgg ttggtactgc
     2761 taataaatca acagtggaag gcattcaggc ctcagtgaag acggcccgag aactcacacc
     2821 ccaggtggtc tcggctgctc gtatcttact taggaaccct ggaaatcaag ctgcttatga
     2881 acattttgag accatgaaga accagtggat cgataatgtt gaaaaaatga cagggctggt
     2941 ggacgaagcc attgatacca aatctctgtt ggatgcttca gaagaagcaa ttaaaaaaga
     3001 cctggacaag tgcaaggtag ctatggccaa cattcagcct cagatgctgg ttgctggggc
     3061 aaccagtatt gctcgtcggg ccaaccggat cctgctggtg gctaagaggg aggtggagaa
     3121 ttccgaggat cccaagttcc gtgaggctgt gaaagctgcc tctgatgaat tgagcaaaac
     3181 catctccccg atggtgatgg atgcaaaagc tgtggctgga aacatttccg accctggact
     3241 gcaaaagagc ttcctggact caggatatcg gatcctggga gctgtggcca aggtcagaga
     3301 agccttccaa cctcaggagc ctgacttccc gccgcctcca ccagaccttg aacaactccg
     3361 actaacagat gagcttgctc ctcccaaacc acctctgcct gaaggtgagg tccctccacc
     3421 taggcctcca ccaccagagg aaaaggatga agagttccct gagcagaagg ccggggaggt
     3481 gattaaccag ccaatgatga tggctgccag acagctccat gatgaagctc gcaaatggtc
     3541 cagcaagggc aatgacatca ttgcagcagc caagcgcatg gctctgctga tggctgagat
     3601 gtctcggctg gtaagagggg gcagtggtac caagcgggca ctcattcagt gtgccaagga
     3661 catcgccaag gcctcagatg aggtgactcg gttggccaag gaggttgcca agcagtgcac
     3721 agataaacgg attagaacca acctcttaca ggtatgtgag cgaatcccaa ccataagcac
     3781 ccagctcaaa atcctgtcca cagtgaaggc caccatgctg ggccggacca acatcagtga
     3841 tgaggagtct gagcaggcca cagagatgct ggttcacaat gcccagaacc tcatgcagtc
     3901 tgtgaaggag actgtgcggg aagctgaagc tgcttcaatc aaaattcgaa cagatgctgg
     3961 atttacactg cgctgggtta gaaagactcc ctggtaccag taggcggccg cgactctaga
     4021 tcataatcag ccataccac
//
