LOCUS       sequence-85351-e        6035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tight Junctions, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6035)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-ZO1-C-14
FEATURES             Location/Qualifiers
     source          1..6035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric derivative of DsRed (Shaner
                     et al., 2008)"
                     /label=mApple
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEA
                     FQTAKLKVTKGGPLPFAWDILSPQFMYGSKVYIKHPADIPDYFKLSFPEGFRWERVMNF
                     EDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPSDGPVMQKKTMGWEASEERMYPEDGALK
                     SEIKKRLKLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     RBS             2443..2451
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgaggcct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg tctacattaa gcacccagcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaggtggga gcgcgtgatg aacttcgagg acggcggcat
      361 tattcacgtt aaccaggact cctccctgca ggacggcgtg ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctccgag gagcggatgt accccgagga cggcgccctg aagagcgaga tcaagaagag
      541 gctgaagctg aaggacggcg gccactacgc cgccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcgtgtccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggaag tgctgggagc gcaagcggaa gcggaggctc
      781 cggtgaggaa acagctatat gggaacaaca tacagtgacg cttcacaggg ctcctggatt
      841 tggatttgga attgcaatat ctggtggacg agataatcct cattttcaga gtggggaaac
      901 gtcaatagtg atttcagatg tgctgaaagg aggaccagct gaaggacagc tacaggaaaa
      961 tgaccgagtt gcaatggtta acggagtttc aatggataat gttgaacatg cttttgctgt
     1021 tcagcaacta aggaaaagtg ggaaaaatgc aaaaattaca attagaagga agaagaaagt
     1081 tcaaatacca gtaagtcgtc ctgatcctga accagtatct gataatgaag aagatagtta
     1141 tgatgaggaa atacatgatc caagaagtgg ccggagtggt gtggttaaca gaaggagtga
     1201 gaagatttgg ccgagggata gaagtgcaag tagagagagg agcttgtccc cgcggtcaga
     1261 caggcggtca gtggcttcca gccagcctgc taaacctact aaagtcacac tggtgaaatc
     1321 ccggaaaaat gaagaatatg gtcttcgatt ggcaagccat atatttgtta aggaaatttc
     1381 acaagatagt ttggcagcaa gagatggcaa tattcaagaa ggtgatgttg tattgaagat
     1441 aaatggtact gtgacagaaa atatgtcatt gacagatgca aagacattga tagaaaggtc
     1501 taaaggcaaa ttaaaaatgg tagttcaaag agatgaacgg gctacgctat tgaatgtccc
     1561 tgatctttct gacagcatcc actctgctaa tgcctctgag agagacgaca tttcagaaat
     1621 tcagtcactg gcatcagatc attctggtcg atcacacgat aggcctcccc gccgcagccg
     1681 gtcacgatct cctgaccagc ggtcagagcc ttctgatcat tccaggcact cgccgcagca
     1741 gccaagcaat ggcagtctgc ggagtagaga tgaagagaga atttctaaac ctggggctgt
     1801 ctcaactcct gtaaagcatg ctgatgatca cacacctaaa acagtggaag aagttacagt
     1861 tgaaagaaat gagaaacaaa caccttctct tccagaacca aagcctgtgt atgcccaagt
     1921 tggcaaccag atgtggattt acctgtcagt ccatctgatg gtgtcctacc taattcaact
     1981 catgaagatg ggatttcttc ggcccagcat gaaattggta aaattcagaa aaggagatag
     2041 tgtgggtttg cggctggctg gtggaaatga tgttggaata tttgtagctg gcgttctaga
     2101 agatagccct gcagccaagg aaggcttaga ggaaggtgat caaattctca gggtaaacaa
     2161 cgtagatttt acaaatatca taagagaaga agccgtcctt ttcctgcttg acctccctaa
     2221 aggagaagaa gtgaccatat tggctcagaa gaagaaggat gtttatcgtc gcattgtaga
     2281 atcagatgta ggagattctt tctatattag aacccatttt gaatatgaaa aggaatctcc
     2341 ctatggactt agttttaaca aaggagaggt gttccgtgct gtggatacct tgtacaatgg
     2401 aaaactgggc tcttggcttg ctattcgaat tggtaaaaat cataaggagg tagaacgagg
     2461 catcatccct aataagaaca gagctgagca gctcgccagt gtacagtata cacttccaaa
     2521 aacagcaggc ggagaccgtg ctgacttctg gagattcaga ggtcttcgca gctccaagag
     2581 aaatcttcga aaaagcagag aggatttgtc cgctcagcct gttcaaacaa agtttccagc
     2641 ttatgaaaga gtggttcttc gagaagctgg atttctgagg cctgtaacca tttttggacc
     2701 aatagctgat gttgccagag aaaagctggc aagagaagaa ccagatattt atcaaattgc
     2761 aaagagtgaa ccacgagacg ctggaactga ccaacgtagc tctggctata ttcgcctgca
     2821 tacaataaag caaatcatag atcaagacaa acatgcttta ttagatgtaa caccaaatgc
     2881 agttgatcgt cttaactatg cccagtggta tccaattgtt gtatttctta accctgattc
     2941 taagcaagga gtaaaaacaa tgagaatgag gttatgtcca gaatctcgga aaagtgccag
     3001 gaagttatac gagcgatctc ataaacttgc taaaaataat caccatcttt ttacaactac
     3061 aattaactta aattcaatga atgatggttg gtatggtgcg ctgaaagaag cagttcaaca
     3121 acagcaaaac cagctggtat gggtttccga gggaaaggcg gatggtgcta caagtgatga
     3181 ccttgatttg catgatgatc gtctgtccta cctgtcagct ccaggtagtg aatactcaat
     3241 gtatagcacg gacagtagac acacttctga ctatgaagac acagacacag aaggcggggc
     3301 ctacactgat caagaactag atgaaactct taatgatgag gttgggactc caccggagtc
     3361 tgccattaca cggtcctctg agcctgtaag agaggactcc tctggaatgc atcatgaaaa
     3421 ccaaacatat cctccttact caccacaagc gcagccacaa ccaattcata gaatagactc
     3481 ccctggattt aagccagcct ctcaacagaa agcagaagct tcatctccag tcccttacct
     3541 ttcgcctgaa acaaacccag catcatcaac ctctgctgtt aatcataatg taaatttaac
     3601 taatgtcaga ctggaggagc ccaccccagc tccttccacc tcttactcac cacaagctga
     3661 ttctttaaga acaccaagta ctgaggcagc tcacataatg ctaagagatc aagaaccatc
     3721 attgtcgtcg catgtagatc caacaaaggt gtatagaaag gacccatatc ccgaggaaat
     3781 gatgaggcag aaccatgttt tgaaacagcc agccgttagt cacccagggc acaggccaga
     3841 caaagagcct aatctgacct atgaacccca actcccatac gtagagaaac aagccagcag
     3901 agacctggag cagcccacat acagatacga gtcctcaagc tatacggacc agttttctcg
     3961 aaactatgaa catcgtctgc gatacgaaga tcgcgtcccc atgtatgaag aacagtggtc
     4021 atattatgat gacaaacagc cctacccatc tcggccacct tttgataatc agcactctca
     4081 agaccttgac tccagacagc atcccgaaga gtcctcagaa cgagggtact ttccacgttt
     4141 tgaagagcca gcccctctgt cttacgacag cagaccacgt tacgaacagg cacctagagc
     4201 atccgccctg cggcacgaag agcagccagc tcctgggtat gacacacatg gtagactcag
     4261 accggaagcc cagccccacc cttcagcagg gcccaagcct gcagagtcca agcagtattt
     4321 tgagcaatat tcacgcagtt acgagcaagt accaccccaa ggatttacct ctagagcagg
     4381 tcattttgag cctctccatg gtgctgcagc tgtccctccg ctgatacctt catctcagca
     4441 taagccagaa gctctgcctt caaacaccaa accactgcct ccacccccaa ctcaaaccga
     4501 agaagaggaa gatccagcaa tgaagccaca gtctgtactc accagagtta agatgtttga
     4561 aaacaaaagg tctgcatcct tagagaccaa gaaggatgta aatgacactg gcagttttaa
     4621 gcctccagaa gtagcatcta aaccttcagg tgctcccatc attggtccca aacccacttc
     4681 tcagaatcaa ttcagtgaac atgacaaaac tctgtacagg attccagaac ctcaaaaacc
     4741 tcaactgaag ccacctgaag atattgttcg gtccaatcat tatgaccctg aagaagatga
     4801 agaatattat cgaaaacagc tgtcatactt tgaccgaaga agttttgaga ataagcctcc
     4861 tgcacacatt gctgccagcc atctctccga gcctgcaaag ccagctcatt ctcagaatca
     4921 atcaaatttt tctagttatt cttcaaaggg aaagcctcct gaagctgatg gtgtggatag
     4981 atcatttggc gagaaacgct atgaacccat ccaggccact ccccctcctc ctccattgcc
     5041 ctcgcagtat gcccagccat ctcagcctgt caccagcgcg tctctccaca tacattctaa
     5101 gggagcacat ggtgaaggta attcagtgtc attggatttt cagaattcct tagtgtccaa
     5161 accagaccca cctccatctc agaataagcc agcaactttc agaccaccaa accgagaaga
     5221 tactgctcag gcagctttct atccccagaa aagttttcca gataaagccc cagttaatgg
     5281 aactgaacag actcagaaaa cagtcactcc agcatacaat cgattcacac caaaaccata
     5341 tacaagttct gcccgaccat ttgaacgcaa gtttgaaagt cctaaattca atcacaatct
     5401 tctgccaagt gaaactgcac ataaacctga cttgtcttca aaaactccca cttctccaaa
     5461 aactcttgtg aaatcgcaca gtttggcaca gcctcctgag tttgacagtg gagttgaaac
     5521 tttctctatc catgcagaga agcctaaata tcaaataaat aatatcagca cagtgcctaa
     5581 agctattcct gtgagtcctt cagctgtgga agaggatgaa gatgaagatg gtcatactgt
     5641 ggtggccaca gcccgaggca tatttaacag caatgggggc gtgctgagtt ccatagaaac
     5701 tggtgttagt ataattatcc ctcaaggagc cattcccgaa ggagttgagc aggaaatcta
     5761 tttcaaggtc tgccgggaca acagcatcct tccaccttta gataaagaga aaggtgaaac
     5821 actgctgagt cctttggtga tgtgtggtcc ccatggcctc aagttcctga agcctgtgga
     5881 gctgcgctta ccacactgtg atcctaaaac ctggcaaaac aagtgtcttc ccggagatcc
     5941 aaattatctc gttggagcaa actgtgtttc tgtccttatt gaccactttt aaggatccac
     6001 cggatctaga taactgatca taatcagcca tacca
//
