LOCUS       sequence-85354-e        1968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Rizzo MA, Davidson MW, Piston DW
  TITLE     Fluorescent protein tracking and detection: fluorescent protein 
            structure and color variants.
  JOURNAL   Cold Spring Harb Protoc. 2009 Dec;2009(12):pdb.top63. doi: 
            10.1101/pdb.top63.
  PUBMED    20150100
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cold Spring
            Harb Protoc."; date: "2009-12"; volume: "2009(12):pdb.top63. doi"; 
            pages: " 10.1101/pdb.top63"
COMMENT     Sequence Label: mCherry-Actin-C-18
FEATURES             Location/Qualifiers
     source          1..1968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctggca gcggtggagg cagcgcatcc
      781 ggcggaagcg gaagcgatga tgatatcgcc gcgctcgtcg tcgacaacgg ctccggcatg
      841 tgcaaggccg gcttcgcggg cgacgatgcc ccccgggccg tcttcccctc catcgtgggg
      901 cgccccaggc accagggcgt gatggtgggc atgggtcaga aggattccta tgtgggcgac
      961 gaggcccaga gcaagagagg catcctcacc ctgaagtacc ccatcgagca cggcatcgtc
     1021 accaactggg acgacatgga gaaaatctgg caccacacct tctacaatga gctgcgtgtg
     1081 gctcccgagg agcaccccgt gctgctgacc gaggcccccc tgaaccccaa ggccaaccgc
     1141 gagaagatga cccagatcat gtttgagacc ttcaacaccc cagccatgta cgttgctatc
     1201 caggctgtgc tatccctgta cgcctctggc cgtaccactg gcatcgtgat ggactccggt
     1261 gacggggtca cccacactgt gcccatctac gaggggtatg ccctccccca tgccatcctg
     1321 cgtctggacc tggctggccg ggacctgact gactacctca tgaagatcct caccgagcgc
     1381 ggctacagct tcaccaccac ggccgagcgg gaaatcgtgc gtgacattaa ggagaagctg
     1441 tgctacgtcg ccctggactt cgagcaagag atggccacgg ctgcttccag ctcctccctg
     1501 gagaagagct acgagctgcc tgacggccag gtcatcacca ttggcaatga gcggttccgc
     1561 tgccctgagg cactcttcca gccttccttc ctgggcatgg agtcctgtgg catccacgaa
     1621 actaccttca actccatcat gaagtgtgac gtggacatcc gcaaagacct gtacgccaac
     1681 acagtgctgt ctggcggcac caccatgtac cctggcattg ccgacaggat gcagaaggag
     1741 atcactgccc tggcacccag cacaatgaag atcaagatca ttgctcctcc tgagcgcaag
     1801 tactccgtgt ggatcggcgg ctccatcctg gcctcgctgt ccaccttcca gcagatgtgg
     1861 atcagcaagc aggagtatga cgagtccggc ccctccatcg tccaccgcaa atgcttctag
     1921 ggatccaccg gatctagata actgatcata atcagccata ccacatga
//
