LOCUS       sequence-85357-e        3988 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3988)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Alpha-5-Integrin-12
FEATURES             Location/Qualifiers
     source          1..3988
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3253..3262
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3259..3969
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3404..3422)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3672..3691
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccaccatggg gagccggacg ccagagtccc ctctccacgc cgtgcagctg
      121 cgctggggcc cccggcgccg acccccgctg ctgccgctgc tgttgctgct gctgccgccg
      181 ccacccaggg tcgggggctt caacttagac gcggaggccc cagcagtact ctcggggccc
      241 ccgggctcct tcttcggatt ctcagtggag ttttaccggc cgggaacaga cggggtcagt
      301 gtgctggtgg gagcacccaa ggctaatacc agccagccag gagtgctgca gggtggtgct
      361 gtctacctct gtccttgggg tgccagcccc acacagtgca cccccattga atttgacagc
      421 aaaggctctc ggctcctgga gtcctcactg tccagctcag agggagagga gcctgtggag
      481 tacaagtcct tgcagtggtt cggggcaaca gttcgagccc atggctcctc catcttggca
      541 tgcgctccac tgtacagctg gcgcacagag aaggagccac tgagcgaccc cgtgggcacc
      601 tgctacctct ccacagataa cttcacccga attctggagt atgcaccctg ccgctcagat
      661 ttcagctggg cagcaggaca gggttactgc caaggaggct tcagtgccga gttcaccaag
      721 actggccgtg tggttttagg tggaccagga agctatttct ggcaaggcca gatcctgtct
      781 gccactcagg agcagattgc agaatcttat taccccgagt acctgatcaa cctggttcag
      841 gggcagctgc agactcgcca ggccagttcc atctatgatg acagctacct aggatactct
      901 gtggctgttg gtgaattcag tggtgatgac acagaagact ttgttgctgg tgtgcccaaa
      961 gggaacctca cttacggcta tgtcaccatc cttaatggct cagacattcg atccctctac
     1021 aacttctcag gggaacagat ggcctcctac tttggctatg cagtggccgc cacagacgtc
     1081 aatggggacg ggctggatga cttgctggtg ggggcacccc tgctcatgga tcggacccct
     1141 gacgggcggc ctcaggaggt gggcagggtc tacgtctacc tgcagcaccc agccggcata
     1201 gagcccacgc ccacccttac cctcactggc catgatgagt ttggccgatt tggcagctcc
     1261 ttgacccccc tgggggacct ggaccaggat ggctacaatg atgtggccat cggggctccc
     1321 tttggtgggg agacccagca gggagtagtg tttgtatttc ctgggggccc aggagggctg
     1381 ggctctaagc cttcccaggt tctgcagccc ctgtgggcag ccagccacac cccagacttc
     1441 tttggctctg cccttcgagg aggccgagac ctggatggca atggatatcc tgatctgatt
     1501 gtggggtcct ttggtgtgga caaggctgtg gtatacaggg gccgccccat cgtgtccgct
     1561 agtgcctccc tcaccatctt ccccgccatg ttcaacccag aggagcggag ctgcagctta
     1621 gaggggaacc ctgtggcctg catcaacctt agcttctgcc tcaatgcttc tggaaaacac
     1681 gttgctgact ccattggttt cacagtggaa cttcagctgg actggcagaa gcagaaggga
     1741 ggggtacggc gggcactgtt cctggcctcc aggcaggcaa ccctgaccca gaccctgctc
     1801 atccagaatg gggctcgaga ggattgcaga gagatgaaga tctacctcag gaacgagtca
     1861 gaatttcgag acaaactctc gccgattcac atcgctctca acttctcctt ggacccccaa
     1921 gccccagtgg acagccacgg cctcaggcca gccctacatt atcagagcaa gagccggata
     1981 gaggacaagg ctcagatctt gctggactgt ggagaagaca acatctgtgt gcctgacctg
     2041 cagctggaag tgtttgggga gcagaaccat gtgtacctgg gtgacaagaa tgccctgaac
     2101 ctcactttcc atgcccagaa tgtgggtgag ggtggcgcct atgaggctga gcttcgggtc
     2161 accgcccctc cagaggctga gtactcagga ctcgtcagac acccagggaa cttctccagc
     2221 ctgagctgtg actactttgc cgtgaaccag agccgcctgc tggtgtgtga cctgggcaac
     2281 cccatgaagg caggagccag tctgtggggt ggccttcggt ttacagtccc tcatctccgg
     2341 gacactaaga aaaccatcca gtttgacttc cagatcctca gcaagaatct caacaactcg
     2401 caaagcgacg tggtttcctt tcggctctcc gtggaggctc aggcccaggt caccctgaac
     2461 ggtgtctcca agcctgaggc agtgctattc ccagtaagcg actggcatcc ccgagaccag
     2521 cctcagaagg aggaggacct gggacctgct gtccaccatg tctatgagct catcaaccaa
     2581 ggccccagct ccattagcca gggtgtgctg gaactcagct gtccccaggc tctggaaggt
     2641 cagcagctcc tatatgtgac cagagttacg ggactcaact gcaccaccaa tcaccccatt
     2701 aacccaaagg gcctggagtt ggatcccgag ggttccctgc accaccagca aaaacgggaa
     2761 gctccaagcc gcagctctgc ttcctcggga cctcagatcc tgaaatgccc ggaggctgag
     2821 tgtttcaggc tgcgctgtga gctcgggccc ctgcaccaac aagagagcca aagtctgcag
     2881 ttgcatttcc gagtctgggc caagactttc ttgcagcggg agcaccagcc atttagcctg
     2941 cagtgtgagg ctgtgtacaa agccctgaag atgccctacc gaatcctgcc tcggcagctg
     3001 ccccaaaaag agcgtcaggt ggccacagct gtgcaatgga ccaaggcaga aggcagctat
     3061 ggcgtcccac tgtggatcat catcctagcc atcctgtttg gcctcctgct cctaggtcta
     3121 ctcatctaca tcctctacaa gcttggattc ttcaaacgct ccctcccata tggcaccgcc
     3181 atggaaaaag ctcagctcaa gcctccagcc acctctgatg ccacggtacc gcgggcccgg
     3241 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     3301 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     3361 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     3421 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     3481 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     3541 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     3601 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     3661 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     3721 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     3781 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3841 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     3901 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     3961 tacaagtaaa gcggccgcga ctctagac
//
