LOCUS       sequence-85358-e        1964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1964)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Alpha-Actin-12
FEATURES             Location/Qualifiers
     source          1..1964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      30..39
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..743
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(181..199)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     449..468
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc gaggaggata
       61 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
      121 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      181 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      241 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
      301 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
      361 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
      421 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
      481 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
      541 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
      601 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
      661 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
      721 gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct tcgaattcta
      781 tgtgtgaaga agaggacagc actgccttgg tgtgtgacaa tggctctggg ctctgtaagg
      841 ccggctttgc tggggacgat gctcccaggg ctgttttccc atccattgtg ggacgtccca
      901 gacatcaggg ggtgatggtg ggaatgggac aaaaagacag ctacgtgggt gacgaagcac
      961 agagcaaaag aggaatcctg accctgaagt acccgataga acatggcatc atcaccaact
     1021 gggacgacat ggaaaagatc tggcaccact ctttctacaa tgagcttcgt gttgcccctg
     1081 aagagcatcc caccctgctc acggaggcac ccctgaaccc caaggccaac cgggagaaaa
     1141 tgactcaaat tatgtttgag actttcaatg tcccagccat gtatgtggct atccaggcgg
     1201 tgctgtctct ctatgcctct ggacgcacaa ctggcatcgt gctggactct ggagatggtg
     1261 tcacccacaa tgtccccatc tatgagggct atgccttgcc ccatgccatc atgcgtctgg
     1321 atctggctgg ccgagatctc actgactacc tcatgaagat cctgactgag cgtggctatt
     1381 ccttcgttac tactgctgag cgtgagattg tccgggacat caaggagaaa ctgtgttatg
     1441 tagctctgga ctttgaaaat gagatggcca ctgccgcatc ctcatcctcc cttgagaaga
     1501 gttacgagtt gcctgatggg caagtgatca ccatcggaaa tgaacgtttc cgctgcccag
     1561 agaccctgtt ccagccatcc ttcatcggga tggagtctgc tggcatccat gaaaccacct
     1621 acaacagcat catgaagtgt gatattgaca tcaggaagga cctctatgct aacaatgtcc
     1681 tatcaggggg caccactatg taccctggca ttgccgaccg aatgcagaag gagatcacgg
     1741 ccctagcacc cagcaccatg aagatcaaga tcattgcccc tccggagcgc aaatactctg
     1801 tctggatcgg tggctccatc ctggcctctc tgtccacctt ccagcagatg tggatcagca
     1861 aacaggaata cgatgaagcc gggccttcca tcgtccaccg caaatgcttc taaggatcca
     1921 ccggatctag ataactgatc ataatcagcc ataccacatt gaga
//
