LOCUS       sequence-85365-e        1800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1800)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Annexin-8
FEATURES             Location/Qualifiers
     source          1..1800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      59..68
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1046..1055
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1052..1762
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1197..1215)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1465..1484
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctcgc
       61 caccatggcc atggcaacca aaggaggtac tgtcaaagct gcttcaggat tcaatgccat
      121 ggaagatgcc cagaccctga ggaaggccat gaaagggctc ggcaccgatg aagacgccat
      181 tattagcgtc cttgcctacc gcaacaccgc ccagcgccag gagatcagga cagcctacaa
      241 gagcaccatc ggcagggact tgatagacga cctgaagtca gaactgagtg gcaacttcga
      301 gcaggtgatt gtggggatga tgacgcccac ggtgctgtat gacgtgcaag agctgcgaag
      361 ggccatgaag ggagccggca ctgatgaggg ctgcctaatt gagatcctgg cctcccggac
      421 ccctgaggag atccggcgca taagccaaac ctaccagcag caatatggac ggagccttga
      481 agatgacatt cgctctgaca catcgttcat gttccagcga gtgctggtgt ctctgtcagc
      541 tggtgggagg gatgaaggaa attatctgga cgatgctctc gtgagacagg atgcccagga
      601 cctgtatgag gctggagaga agaaatgggg gacagatgag gtgaaatttc taactgttct
      661 ctgttcccgg aaccgaaatc acctgttgca tgtgtttgat gaatacaaaa ggatatcaca
      721 gaaggatatt gaacagagta ttaaatctga aacatctggt agctttgaag atgctctgct
      781 ggctatagta aagtgcatga ggaacaaatc tgcatatttt gctgaaaagc tctataaatc
      841 gatgaagggc ttgggcaccg atgataacac cctcatcaga gtgatggttt ctcgagcaga
      901 aattgacatg ttggatatcc gggcacactt caagagactc tatggaaagt ctctgtactc
      961 gttcatcaag ggtgacacat ctggagacta caggaaagta ctgcttgttc tctgtggagg
     1021 agatgattct cgggatccac cggtcgccac catggtgagc aagggcgagg aggataacat
     1081 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     1141 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     1201 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     1261 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     1321 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     1381 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     1441 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     1501 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     1561 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     1621 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     1681 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     1741 catggacgag ctgtacaagt aaagcggccg cgactctaga tcataatcag ccataccaca
//
