LOCUS       sequence-85366-e        2018 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2018)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ARP2-C-14
FEATURES             Location/Qualifiers
     source          1..2018
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(173..191)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     441..460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
       61 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      181 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      241 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      301 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      361 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      481 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
      541 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
      661 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagtccgg aagtgctggg agcgcaagcg gaagcggagg ctccggtgac
      781 agccagggca ggaaggtggt ggtgtgcgac aacggcaccg ggtttgtgaa gtgtggatat
      841 gcaggctcta actttccaga acacatcttc ccagctttgg ttggaagacc tattatcaga
      901 tcaaccacca aagtgggaaa cattgaaatc aagaataaca aaaagatgga tcttatggtt
      961 ggtgatgagg caagtgaatt acgatcaatg ttagaagtta actaccctat ggaaaatggc
     1021 atagtacgaa attgggatga catgaaacac ctgtgggact acacatttgg accagagaaa
     1081 cttaatatag ataccagaaa ttgtaaaatc ttactcacag aacctcctat gaacccaacc
     1141 aaaaacagag agaagattgt agaggtaatg tttgaaactt accagttttc cggtgtatat
     1201 gtagccatcc aggcagttct gactttgtac gctcaaggtt tattgactgg tgtagtggta
     1261 gactctggag atggtgtgac tcacatttgc ccagtatatg aaggcttttc tctccctcat
     1321 cttaccagga gactggatat tgctgggagg gatataacta gatatcttat caagctactt
     1381 ctgttgcgag gatacgcctt caaccactct gctgattttg aaacggttcg catgattaaa
     1441 gaaaaactgt gttacgtggg atataatatt gagcaagagc agaaactggc cttagaaacc
     1501 acagtattag ttgaatctta tacactccca gatggacgta tcatcaaagt tgggggagag
     1561 agatttgaag caccagaagc tttatttcag cctcacttga tcaatgttga aggagttggt
     1621 gttgctgaat tgctttttaa cacaattcag gcagctgaca ttgataccag atctgaattc
     1681 tacaaacaca ttgtgctttc tggagggtct actatgtatc ctggcctgcc atcacggttg
     1741 gaacgagaac ttaaacagct ttacttagaa cgagttttga agggtgatgt ggaaaaactt
     1801 tctaaattta agatccgcat tgaagaccca ccccgcagaa agcacatggt attcctgggt
     1861 ggtgcagttc tagcggatat catgaaagac aaagacaact tttggatgac ccgacaagag
     1921 taccaagaaa agggtgtccg tgtgctagag aaacttggtg tgactgttcg ataaggatcc
     1981 accggatcta gataactgat cataatcagc cataacac
//
