LOCUS       sequence-85367-e        2019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2019)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ARP2-N-14
FEATURES             Location/Qualifiers
     source          1..2019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1270..1279
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1276..1986
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1421..1439)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1689..1708
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgctagcgc taccggactc agatctcgag accgccatgg acagccaggg caggaaggtg
       61 gtggtgtgcg acaacggcac cgggtttgtg aagtgtggat atgcaggctc taactttcca
      121 gaacacatct tcccagcttt ggttggaaga cctattatca gatcaaccac caaagtggga
      181 aacattgaaa tcaagaataa caaaaagatg gatcttatgg ttggtgatga ggcaagtgaa
      241 ttacgatcaa tgttagaagt taactaccct atggaaaatg gcatagtacg aaattgggat
      301 gacatgaaac acctgtggga ctacacattt ggaccagaga aacttaatat agataccaga
      361 aattgtaaaa tcttactcac agaacctcct atgaacccaa ccaaaaacag agagaagatt
      421 gtagaggtaa tgtttgaaac ttaccagttt tccggtgtat atgtagccat ccaggcagtt
      481 ctgactttgt acgctcaagg tttattgact ggtgtagtgg tagactctgg agatggtgtg
      541 actcacattt gcccagtata tgaaggcttt tctctccctc atcttaccag gagactggat
      601 attgctggga gggatataac tagatatctt atcaagctac ttctgttgcg aggatacgcc
      661 ttcaaccact ctgctgattt tgaaacggtt cgcatgatta aagaaaaact gtgttacgtg
      721 ggatataata ttgagcaaga gcagaaactg gccttagaaa ccacagtatt agttgaatct
      781 tatacactcc cagatggacg tatcatcaaa gttgggggag agagatttga agcaccagaa
      841 gctttatttc agcctcactt gatcaatgtt gaaggagttg gtgttgctga attgcttttt
      901 aacacaattc aggcagctga cattgatacc agatctgaat tctacaaaca cattgtgctt
      961 tctggagggt ctactatgta tcctggcctg ccatcacggt tggaacgaga acttaaacag
     1021 ctttacttag aacgagtttt gaagggtgat gtggaaaaac tttctaaatt taagatccgc
     1081 attgaagacc caccccgcag aaagcacatg gtattcctgg gtggtgcagt tctagcggat
     1141 atcatgaaag acaaagacaa cttttggatg acccgacaag agtaccaaga aaagggtgtc
     1201 cgtgtgctag agaaacttgg tgtgactgtt cgagggggtg cgtcaggcgg cagcggatct
     1261 ggaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag
     1321 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     1381 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     1441 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     1501 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     1561 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     1621 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     1681 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     1741 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     1801 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     1861 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     1921 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     1981 aagtaaagcg gccgcgactc tagatcataa tcagccata
//
