LOCUS       sequence-85368-e        2036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2036)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ARP3-C-12
FEATURES             Location/Qualifiers
     source          1..2036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             17..724
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(162..180)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     430..449
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
       61 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
      121 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
      181 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
      241 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
      301 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
      361 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
      421 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
      481 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
      541 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
      601 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
      661 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
      721 caagtccgga ctcagatctg gtggaagcgg cgctggaggg gcgggacggc tgccggcctg
      781 tgtggtggac tgtggcacgg ggtatacaaa actaggatat gctggaaata cagaaccaca
      841 gtttatcatc ccttcctgta ttgctattaa ggagtcagca aaagtgggtg atcaagctca
      901 aaggagggtg atgaaaggtg ttgatgacct agacttcttc attggtgatg aagcaataga
      961 aaaacctaca tatgcaacaa agtggccaat ccgccatggt atagttgaag attgggactt
     1021 aatggaaagg tttatggagc aagtgatctt taaatattta agggcagaac ctgaagacca
     1081 ttattttctt ttgactgaac ctccattgaa tactccagaa aacagggaat atactgctga
     1141 aataatgttt gagtccttca atgttccagg cttgtacatt gctgtgcagg ctgttcttgc
     1201 cttagctgca tcttggacct caagacaagt aggagaacgg acgttgaccg gtacggtaat
     1261 agacagtgga gatggtgtca ctcatgtcat tcctgtggct gaagggtatg tgattggcag
     1321 ctgtattaaa cacattccaa tcgcaggacg agatataaca tattttattc agcaactgct
     1381 gagagaccga gaagtaggaa tccctccaga acaatccttg gaaactgcta aggcagtaaa
     1441 ggagcgctat agttatgtct gcccagattt agtaaaagaa tttaacaagt atgatacaga
     1501 tgggtcaaaa tggattaaac agtatactgg aatcaatgct atctcaaaga aagagttttc
     1561 tatcgatgtt ggttatgaga gatttttggg acctgaaatc ttttttcatc cagagtttgc
     1621 taatccagac tttacacaac ctatctcaga agttgtagat gaagtaattc agaattgtcc
     1681 tattgatgtc agacgtcctc tctacaagaa tattgtcctc tctggaggtt caaccatgtt
     1741 cagggacttt ggacgtcgct tgcaaagaga tttgaaaaga actgtagatg cccggctgaa
     1801 attaagtgag gaattgagtg gtggtagatt gaagccaaaa cctattgatg tacaagtcat
     1861 tacacaccac atgcagcgat atgcagtttg gtttggagga tcaatgctgg cttccacgcc
     1921 tgagttctac caagtatgcc acaccaaaaa ggattatgaa gaaattggac ctagcatttg
     1981 tcgtcacaat ccagtgtttg gagtcatgtc gtaaggatcc accggatcta gataac
//
