LOCUS       sequence-85369-e        2046 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2046)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2046)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ARP3-N-12
FEATURES             Location/Qualifiers
     source          1..2046
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1300..1309
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1306..2016
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1451..1469)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1719..1738
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccgctagcag ccaccatggc gggacggctg ccggcctgtg tggtggactg tggcacgggg
       61 tatacaaaac taggatatgc tggaaataca gaaccacagt ttatcatccc ttcctgtatt
      121 gctattaagg agtcagcaaa agtgggtgat caagctcaaa ggagggtgat gaaaggtgtt
      181 gatgacctag acttcttcat tggtgatgaa gcaatagaaa aacctacata tgcaacaaag
      241 tggccaatcc gccatggtat agttgaagat tgggacttaa tggaaaggtt tatggagcaa
      301 gtgatcttta aatatttaag ggcagaacct gaagaccatt attttctttt gactgaacct
      361 ccattgaata ctccagaaaa cagggaatat actgctgaaa taatgtttga gtccttcaat
      421 gttccaggct tgtacattgc tgtgcaggct gttcttgcct tagctgcatc ttggacctca
      481 agacaagtag gagaacggac gttgaccggt acggtaatag acagtggaga tggtgtcact
      541 catgtcattc ctgtggctga agggtatgtg attggcagct gtattaaaca cattccaatc
      601 gcaggacgag atataacata ttttattcag caactgctga gagaccgaga agtaggaatc
      661 cctccagaac aatccttgga aactgctaag gcagtaaagg agcgctatag ttatgtctgc
      721 ccagatttag taaaagaatt taacaagtat gatacagatg ggtcaaaatg gattaaacag
      781 tatactggaa tcaatgctat ctcaaagaaa gagttttcta tcgatgttgg ttatgagaga
      841 tttttgggac ctgaaatctt ttttcatcca gagtttgcta atccagactt tacacaacct
      901 atctcagaag ttgtagatga agtaattcag aattgtccta ttgatgtcag acgtcctctc
      961 tacaagaata ttgtcctctc tggaggttca accatgttca gggactttgg acgtcgcttg
     1021 caaagagatt tgaaaagaac tgtagatgcc cggctgaaat taagtgagga attgagtggt
     1081 ggtagattga agccaaaacc tattgatgta caagtcatta cacaccacat gcagcgatat
     1141 gcagtttggt ttggaggatc aatgctggct tccacgcctg agttctacca agtatgccac
     1201 accaaaaagg attatgaaga aattggacct agcatttgtc gtcacaatcc agtgtttgga
     1261 gtcatgtcgg catccggcgg tagcggggat ccaccggtcg ccaccatggt gagcaagggc
     1321 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
     1381 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     1441 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
     1501 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
     1561 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     1621 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
     1681 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
     1741 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
     1801 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
     1861 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
     1921 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
     1981 cactccaccg gcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa
     2041 tcagcc
//
