LOCUS       sequence-85370-e        1717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1717)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ARPp34-C-14
FEATURES             Location/Qualifiers
     source          1..1717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..735
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(173..191)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     441..460
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag cgctaccggt cgccaccatg gtgagcaagg gcgaggagga taacatggcc
       61 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      121 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
      181 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      241 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
      301 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
      361 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
      421 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
      481 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
      541 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
      601 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
      661 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
      721 gacgagctgt acaagtccgg aagtgctggg agcgcaagcg gaagcggagg ctccggtatc
      781 ctgctggagg tgaacaaccg catcatcgag gagacgctcg cgctcaagtt cgagaacgca
      841 gccgccggaa acaaaccgga agcagtagaa gtaacatttg cagatttcga tggggtcctc
      901 tatcatattt caaatcctaa tggagacaaa acaaaagtga tggtcagtat ttctttgaaa
      961 ttctacaagg aacttcaggc acatggtgct gatgagttat taaagagggt gtacgggagt
     1021 ttcttggtaa atccagaatc aggatacaat gtctctttgc tatatgacct tgaaaatctt
     1081 ccggcatcca aggattccat tgtgcatcaa gctggcatgt tgaagcgaaa ttgttttgcc
     1141 tctgtctttg aaaaatactt ccaattccaa gaagagggca aggaaggaga gaacagggca
     1201 gttatccatt atagggatga tgagaccatg tatgttgagt ctaaaaagga cagagtcaca
     1261 gtagtcttca gcacagtgtt taaggatgac gacgatgtgg tcattggaaa ggtgttcatg
     1321 caggagttca aagaaggacg cagagccagc cacacagccc cacaggtcct ctttagccac
     1381 agggaacctc ctctggagct gaaagacaca gacgccgctg tgggtgacaa cattggctac
     1441 attacctttg tgctgttccc tcgtcacacc aatgccagtg cacgagacaa caccatcaac
     1501 ctgatccaca cgttccggga ctacctgcac taccacatca agtgctctaa ggcctatatt
     1561 cacacacgta tgcgggcgaa aacgtctgac ttcctcaagg tgctgaaccg cgcacgccca
     1621 gatgccgaga aaaaagaaat gaaaacaatc acggggaaga cgttttcatc ccgctaagga
     1681 tccaccggat ctagataact gatcataatc agccata
//
