LOCUS       sequence-85371-e        1717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Related Protein, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1717)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ARPp34-N-14
FEATURES             Location/Qualifiers
     source          1..1717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      971..980
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             977..1687
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1122..1140)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1390..1409
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgagac cgccatgatc ctgctggagg tgaacaaccg
       61 catcatcgag gagacgctcg cgctcaagtt cgagaacgca gccgccggaa acaaaccgga
      121 agcagtagaa gtaacatttg cagatttcga tggggtcctc tatcatattt caaatcctaa
      181 tggagacaaa acaaaagtga tggtcagtat ttctttgaaa ttctacaagg aacttcaggc
      241 acatggtgct gatgagttat taaagagggt gtacgggagt ttcttggtaa atccagaatc
      301 aggatacaat gtctctttgc tatatgacct tgaaaatctt ccggcatcca aggattccat
      361 tgtgcatcaa gctggcatgt tgaagcgaaa ttgttttgcc tctgtctttg aaaaatactt
      421 ccaattccaa gaagagggca aggaaggaga gaacagggca gttatccatt atagggatga
      481 tgagaccatg tatgttgagt ctaaaaagga cagagtcaca gtagtcttca gcacagtgtt
      541 taaggatgac gacgatgtgg tcattggaaa ggtgttcatg caggagttca aagaaggacg
      601 cagagccagc cacacagccc cacaggtcct ctttagccac agggaacctc ctctggagct
      661 gaaagacaca gacgccgctg tgggtgacaa cattggctac attacctttg tgctgttccc
      721 tcgtcacacc aatgccagtg cacgagacaa caccatcaac ctgatccaca cgttccggga
      781 ctacctgcac taccacatca agtgctctaa ggcctatatt cacacacgta tgcgggcgaa
      841 aacgtctgac ttcctcaagg tgctgaaccg cgcacgccca gatgccgaga aaaaagaaat
      901 gaaaacaatc acggggaaga cgttttcatc ccgcgggggt gcgtcaggcg gcagcggatc
      961 tggaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     1021 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     1081 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     1141 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     1201 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     1261 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     1321 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     1381 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     1441 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     1501 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     1561 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     1621 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     1681 caagtaaagc ggccgcgact ctagatcata atcagcc
//
