LOCUS       sequence-85372-e        1244 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1244)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1244)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG12-C-18
FEATURES             Location/Qualifiers
     source          1..1244
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat
       61 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      541 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      601 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      721 gctgtacaag tccggactca gatctagtag cggaggtagc gcatccggcg gaagcggagg
      781 aagcgcggag gagccgcagt ctgtgttgca gcttcctact tcaattgctg ctggagggga
      841 aggacttacg gatgtctccc cagaaacaac caccccggag cccccgtctt ccgctgcagt
      901 ttccccggga acagaggaac ctgctggcga caccaagaaa aaaattgaca ttttgctaaa
      961 ggctgtggga gacactccta ttatgaaaac aaagaagtgg gcagtagagc gaacacgaac
     1021 catccaagga ctcattgact tcatcaaaaa gtttcttaaa cttgtggcct cagaacagtt
     1081 gtttatttat gtgaatcagt cctttgctcc ttccccagac caagaagttg gaactctcta
     1141 tgagtgtttt ggcagtgatg gtaaactggt tttacattac tgcaagtctc aggcgtgggg
     1201 atgaggatcc accggatcta gataactgat cataatcagc cata
//
