LOCUS       sequence-85373-e        1273 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1273)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG12-N-18
FEATURES             Location/Qualifiers
     source          1..1273
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      515..524
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             521..1231
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(666..684)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     934..953
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag gccaccatgg cggaggagcc
       61 gcagtctgtg ttgcagcttc ctacttcaat tgctgctgga ggggaaggac ttacggatgt
      121 ctccccagaa acaaccaccc cggagccccc gtcttccgct gcagtttccc cgggaacaga
      181 ggaacctgct ggcgacacca agaaaaaaat tgacattttg ctaaaggctg tgggagacac
      241 tcctattatg aaaacaaaga agtgggcagt agagcgaaca cgaaccatcc aaggactcat
      301 tgacttcatc aaaaagtttc ttaaacttgt ggcctcagaa cagttgttta tttatgtgaa
      361 tcagtccttt gctccttccc cagaccaaga agttggaact ctctatgagt gttttggcag
      421 tgatggtaaa ctggttttac attactgcaa gtctcaggcg tggggaggca gcggtggagg
      481 cagcgcatcc ggcggaagcg gaagcggacc ggtcgccacc atggtgagca agggcgagga
      541 ggataacatg gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt
      601 gaacggccac gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca
      661 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc
      721 ccctcagttc atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta
      781 cttgaagctg tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg
      841 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt
      901 gaagctgcgc ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg
      961 ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa
     1021 gcagaggctg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
     1081 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac
     1141 ctcccacaac gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc
     1201 caccggcggc atggacgagc tgtacaagta aagcggccgc gactctagat cataatcagc
     1261 cataccacat tgt
//
