LOCUS       sequence-85374-e        2380 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2380)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG13-C-18
FEATURES             Location/Qualifiers
     source          1..2380
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..738
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(176..194)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     444..463
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga ggataacatg
       61 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
      121 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
      181 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
      241 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
      301 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
      361 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      421 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
      481 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
      541 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
      601 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
      661 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
      721 atggacgagc tgtacaagtc cggactcaga tctagtagcg gaggtagcgc atccggcgga
      781 agcggaggaa gcgaaactga tctcaattcc caggacagaa aggacctgga caagtttatt
      841 aaattttttg ccctcaagac tgtccaagtg attgtccagg ctcggcttgg tgaaaagatt
      901 tgcactcgtt catcatcttc tccaacgggt tcagattggt tcaacttagc aatcaaagac
      961 atcccagagg ttacacatga agcaaagaag gcactggcag gacagctgcc tgcagtcggg
     1021 aggtccatgt gtgtggagat ttcacttaag acttctgagg gagattccat ggagctggaa
     1081 atatggtgtc ttgaaatgaa tgaaaagtgt gataaagaaa tcaaagtttc ctacacggtg
     1141 tacaacagac tgtcattgct gctgaagtcc cttcttgcta taactagggt gacaccagcc
     1201 tataggctct ccaggaaaca agggcatgaa tatgtcatat tatacaggat atattttgga
     1261 gaagttcagc tgagtggctt aggagaaggc ttccagacag ttcgtgttgg gacagtgggc
     1321 acccctgtgg gcaccatcac tctttcttgt gcttacagaa ttaacttggc attcatgtct
     1381 accaggcaat ttgagaggac cccacctatc atggggatta ttattgatca ctttgtggac
     1441 cgtccctatc ccagctcctc tcccatgcac ccctgcaatt acagaactgc tggtgaggac
     1501 actggagtaa tatacccgtc tgtagaagac tctcaagaag tgtgtaccac ctctttttcc
     1561 acctccccac catcccagct ctcaagctct cgcctttcct atcagcctgc tgccctgggc
     1621 gttggatcag ctgacctggc ttatccagta gtgtttgctg ctggcttaaa tgctacacac
     1681 cctcaccagc tgatggttcc tgggaaggaa ggtggggtac cccttgctcc caaccagcct
     1741 gtccatggta cccaggctga ccaggagaga ctggcaacct gcaccccttc tgacagaacc
     1801 cactgtgctg ccacaccctc cagtagtgag gatactgaaa ccgtatcaaa cagcagtgag
     1861 ggacgggcct cccctcacga tgtcttggag accatctttg tccgaaaagt gggggctttt
     1921 gtcaacaaac ccattaacca ggtgaccctg acgagtttgg atataccctt tgccatgttt
     1981 gctcccaaga atttggagct ggaggatacc gatccaatgg tgaatcctcc agattcccca
     2041 gagactgaat ctcctctcca gggcagcctg cactcagatg gctccagcgg gggcagcagt
     2101 ggcaataccc atgatgactt tgttatgata gactttaaac cagctttttc taaagatgac
     2161 attcttccga tggacctggg gaccttctat cgggagtttc agaacccacc tcagctgagc
     2221 agcctctcca tagatattgg agcacagtcc atggctgaag acttggactc attaccagag
     2281 aagctggctg tgcatgagaa gaatgtccgc gagtttgatg cctttgtgga aaccctgcag
     2341 taaggatcca ccggatctag ataactgatc ataatcagcc
//
