LOCUS       sequence-85375-e        2393 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2393)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG13-N-18
FEATURES             Location/Qualifiers
     source          1..2393
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1626..1635
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1632..2342
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1777..1795)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2045..2064
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ttgacgtcag atccgctagc gccaccatgg aaactgatct caattcccag gacagaaagg
       61 acctggacaa gtttattaaa ttttttgccc tcaagactgt ccaagtgatt gtccaggctc
      121 ggcttggtga aaagatttgc actcgttcat catcttctcc aacgggttca gattggttca
      181 acttagcaat caaagacatc ccagaggtta cacatgaagc aaagaaggca ctggcaggac
      241 agctgcctgc agtcgggagg tccatgtgtg tggagatttc acttaagact tctgagggag
      301 attccatgga gctggaaata tggtgtcttg aaatgaatga aaagtgtgat aaagaaatca
      361 aagtttccta cacggtgtac aacagactgt cattgctgct gaagtccctt cttgctataa
      421 ctagggtgac accagcctat aggctctcca ggaaacaagg gcatgaatat gtcatattat
      481 acaggatata ttttggagaa gttcagctga gtggcttagg agaaggcttc cagacagttc
      541 gtgttgggac agtgggcacc cctgtgggca ccatcactct ttcttgtgct tacagaatta
      601 acttggcatt catgtctacc aggcaatttg agaggacccc acctatcatg gggattatta
      661 ttgatcactt tgtggaccgt ccctatccca gctcctctcc catgcacccc tgcaattaca
      721 gaactgctgg tgaggacact ggagtaatat acccgtctgt agaagactct caagaagtgt
      781 gtaccacctc tttttccacc tccccaccat cccagctctc aagctctcgc ctttcctatc
      841 agcctgctgc cctgggcgtt ggatcagctg acctggctta tccagtagtg tttgctgctg
      901 gcttaaatgc tacacaccct caccagctga tggttcctgg gaaggaaggt ggggtacccc
      961 ttgctcccaa ccagcctgtc catggtaccc aggctgacca ggagagactg gcaacctgca
     1021 ccccttctga cagaacccac tgtgctgcca caccctccag tagtgaggat actgaaaccg
     1081 tatcaaacag cagtgaggga cgggcctccc ctcacgatgt cttggagacc atctttgtcc
     1141 gaaaagtggg ggcttttgtc aacaaaccca ttaaccaggt gaccctgacg agtttggata
     1201 taccctttgc catgtttgct cccaagaatt tggagctgga ggataccgat ccaatggtga
     1261 atcctccaga ttccccagag actgaatctc ctctccaggg cagcctgcac tcagatggct
     1321 ccagcggggg cagcagtggc aatacccatg atgactttgt tatgatagac tttaaaccag
     1381 ctttttctaa agatgacatt cttccgatgg acctggggac cttctatcgg gagtttcaga
     1441 acccacctca gctgagcagc ctctccatag atattggagc acagtccatg gctgaagact
     1501 tggactcatt accagagaag ctggctgtgc atgagaagaa tgtccgcgag tttgatgcct
     1561 ttgtggaaac cctgcagagc agcggtggag caagcgcagc cagtggtagc gcggatccac
     1621 cggtcgccac catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca
     1681 tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
     1741 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg
     1801 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct
     1861 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
     1921 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
     1981 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
     2041 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
     2101 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
     2161 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
     2221 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
     2281 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
     2341 aaagcggccg cgactctaga tcataatcag ccataccaca tttgtagaag ttt
//
