LOCUS       sequence-85376-e        2336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2336)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG14-C-18
FEATURES             Location/Qualifiers
     source          1..2336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat
       61 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      541 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      601 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      721 gctgtacaag tccggactca gatctggcag cggtggaggc agcgcatccg gcggaagcgg
      781 aagcgcgtct cccagtggga agggagcccg ggcgctggag gctcctggct gcgggccccg
      841 gccgctcgcc cgggacctgg tggactccgt ggacgatgcg gaggggctgt acgtggctgt
      901 ggagcgctgc ccgctgtgca acactacccg ccggcggctg acctgcgcca aatgcgttca
      961 gagcggcgat ttcgtctact tcgacggccg cgaccgggag aggtttatcg acaagaagga
     1021 aaggttaagc cgacttaaga gcaagcaaga agaatttcag aaagaagtgt taaaagctat
     1081 ggaaggaaaa tggataacag atcagttgag atggaaaata atgtcctgca agatgaggat
     1141 tgaacagtta aaacaaacaa tatgtaaagg aaatgaagaa atggagaaaa attctgaagg
     1201 ccttctcaaa accaaggaaa agaatcagaa gctttacagt cgagcacaac ggcaccaaga
     1261 gaaaaaggag aagattcaga ggcataatcg caaacttggt gacctggtag aaaaaaagac
     1321 cattgactta agaagtcatt atgagcgtct ggcaaatctt cgacgatccc atatattaga
     1381 gctcacctct gtcatttttc caatcgagga agtaaagacg ggtgtgagag accccgcaga
     1441 tgtgtcttca gagagtgaca gtgccatgac ctccagcact gtgagcaagc ttgctgaagc
     1501 ccggaggaca acttacctct caggacgatg ggtctgtgac gatcacaacg gagacaccag
     1561 cattagcatt acagggcctt ggattagcct ccctaacaat ggggactact ctgcctacta
     1621 cagctgggtg gaggagaaga aaacaaccca ggggcctgac atggagcaga gtaaccctgc
     1681 ctacaccatc agtgctgcgc tgtgctatgc aactcagctg gtcaacattc tgtctcatat
     1741 acttgatgta aatcttccca aaaagctctg caacagtgaa ttttgtggcg aaaatctaag
     1801 caagcagaaa tttactcgag cagtgaagaa actgaatgca aatattcttt acctttgttt
     1861 ttctcagcat gtaaatttag atcaattaca accactgcat accctcagga atctaatgta
     1921 cctggtcagt ccaagctctg aacacctagg caggtcaggg ccctttgaag tacgagcaga
     1981 ccttgaggag tccatggaat ttgtggatcc cggagttgct ggagaatcag atgagagcgg
     2041 agatgagcgc gtcagcgatg aagaaaccga cctgggcaca gactgggaga acttgcctag
     2101 tccccggttt tgtgatatcc cttcccagtc tgtggaagtc tcccagagtc agagcaccca
     2161 ggcgtcccca cccatcgcga gcagcagtgc aggtgggatg atctcctctg cagcagcctc
     2221 ggtgacctcc tggtttaaag cttacactgg acaccgttaa gaattctgca gtcgacggta
     2281 ccgcgggccc gggatccacc ggatctagat aactgatcat aatcagccat accaca
//
