LOCUS       sequence-85377-e        2330 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2330)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG14-N-18
FEATURES             Location/Qualifiers
     source          1..2330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1560..1569
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1566..2276
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1711..1729)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1979..1998
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcaggctag tgacgtcaga tccgctagca ccgccatggc gtctcccagt gggaagggag
       61 cccgggcgct ggaggctcct ggctgcgggc cccggccgct cgcccgggac ctggtggact
      121 ccgtggacga tgcggagggg ctgtacgtgg ctgtggagcg ctgcccgctg tgcaacacta
      181 cccgccggcg gctgacctgc gccaaatgcg ttcagagcgg cgatttcgtc tacttcgacg
      241 gccgcgaccg ggagaggttt atcgacaaga aggaaaggtt aagccgactt aagagcaagc
      301 aagaagaatt tcagaaagaa gtgttaaaag ctatggaagg aaaatggata acagatcagt
      361 tgagatggaa aataatgtcc tgcaagatga ggattgaaca gttaaaacaa acaatatgta
      421 aaggaaatga agaaatggag aaaaattctg aaggccttct caaaaccaag gaaaagaatc
      481 agaagcttta cagtcgagca caacggcacc aagagaaaaa ggagaagatt cagaggcata
      541 atcgcaaact tggtgacctg gtagaaaaaa agaccattga cttaagaagt cattatgagc
      601 gtctggcaaa tcttcgacga tcccatatat tagagctcac ctctgtcatt tttccaatcg
      661 aggaagtaaa gacgggtgtg agagaccccg cagatgtgtc ttcagagagt gacagtgcca
      721 tgacctccag cactgtgagc aagcttgctg aagcccggag gacaacttac ctctcaggac
      781 gatgggtctg tgacgatcac aacggagaca ccagcattag cattacaggg ccttggatta
      841 gcctccctaa caatggggac tactctgcct actacagctg ggtggaggag aagaaaacaa
      901 cccaggggcc tgacatggag cagagtaacc ctgcctacac catcagtgct gcgctgtgct
      961 atgcaactca gctggtcaac attctgtctc atatacttga tgtaaatctt cccaaaaagc
     1021 tctgcaacag tgaattttgt ggcgaaaatc taagcaagca gaaatttact cgagcagtga
     1081 agaaactgaa tgcaaatatt ctttaccttt gtttttctca gcatgtaaat ttagatcaat
     1141 tacaaccact gcataccctc aggaatctaa tgtacctggt cagtccaagc tctgaacacc
     1201 taggcaggtc agggcccttt gaagtacgag cagaccttga ggagtccatg gaatttgtgg
     1261 atcccggagt tgctggagaa tcagatgaga gcggagatga gcgcgtcagc gatgaagaaa
     1321 ccgacctggg cacagactgg gagaacttgc ctagtccccg gttttgtgat atcccttccc
     1381 agtctgtgga agtctcccag agtcagagca cccaggcgtc cccacccatc gcgagcagca
     1441 gtgcaggtgg gatgatctcc tctgcagcag cctcggtgac ctcctggttt aaagcttaca
     1501 ctggacaccg tagcagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg
     1561 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     1621 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1681 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     1741 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     1801 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     1861 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     1921 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     1981 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     2041 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     2101 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     2161 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2221 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaagcg
     2281 gccgcgactc tagatcataa tcagccatac cacatttgta gagatttgct
//
