LOCUS       sequence-85378-e        2627 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2627)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG16-C-18
FEATURES             Location/Qualifiers
     source          1..2627
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(191..209)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     459..478
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccattgctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      121 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      181 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      241 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      301 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      481 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      541 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      601 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      661 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctcg agctcaagct
      781 tcgggagata gtggtgggag cggttcatcg tcgggcctgc gcgccgcaga ctttccccgc
      841 tggaagcgtc acatcgcgga ggaactgagg cgccgggacc gactgcagag gcaggcgttc
      901 gaggagatca ttctgcagta taccaagttg ctggaaaagt cagatcttca ttcagtattg
      961 acccagaaac tacaagcaga aaagcatgac atgccaaata ggcatgaaat aagtcctgga
     1021 catgatggtg cgtggaatga tagtcaacta caagaaatgg cccagttgag gatcaaacac
     1081 caggaagagc tgaccgaact gcacaagaag cgtggggagt tagctcagtt ggtgattgac
     1141 ctgaacaacc aaatgcagca gaaggacaag gagatacaga tgaatgaagc aaagatttcg
     1201 gagtatttac agaccatctc tgacctggag acaaactgcc tggacctgcg caccaaactg
     1261 caggacctcg aggtagccaa ccagaccctg aaggatgagt atgacgccct gcagattact
     1321 tttactgccc tagaagagaa actgaggaaa actactgagg agaaccagga actggtcacc
     1381 agatggatgg ctgagaaggc ccaagaagcc aatcgcctca atgcagagaa tgagaaggac
     1441 tccaggaggc gtcaagcacg gctgcagaag gagcttgcag aagcagcaaa ggaacctcta
     1501 cctgttgaac aggatgatga cattgaagtc attgtggatg agacctcaga ccacacagaa
     1561 gagacctctc ccgtccgagc tgtcagcaga gcagctacga gacgctctgt ctcttccatc
     1621 ccagtccccc aggatatcat ggacactcat cctgcttctg gtaaagatgt gagagtccca
     1681 actactgcct cgtatgtctt cgatgcgcat gacggagagg tcaacgcagt gcagttcagt
     1741 ccaggctccc ggttgctggc cactggaggc atggaccgca gggtgaaact ttgggaagca
     1801 ttcggagata aatgtgaatt caagggctcc ctgtctggca gtaatgctgg aattacaagc
     1861 attgaatttg atagtgctgg agcttaccta ttagcagctt caaatgattt tgcaagccga
     1921 atctggactg tggatgatta tcgattacgg cacacactca caggccacag cgggaaagtc
     1981 ctctctgcca agttcctgct ggacaatgca cggattgtct caggaagtca cgaccggacc
     2041 ctcaaactct gggatctccg cagcaaagtc tgcataaaaa cagtgtttgc aggatccagc
     2101 tgcaatgaca ttgtttgcac tgaacaatgt gtaatgagtg gacattttga caagaaaatt
     2161 cgtttctggg atatccggtc agagagtgtg gtccgagaga tggaactgtt agggaagatc
     2221 actgctctgg acctaaaccc tgagagaact gagctcctga gctgctcccg tgatgacctg
     2281 ctaaaagtca tcgacctccg gacaaatgca gtcaaacaga cattcagtgc gcctggattc
     2341 aaatgcggct ctgactggac ccgggttgtc ttcagccctg atggcagtta cgtggcagca
     2401 ggctcagccg agggttctct ttatgtctgg agtgtgctga cagggaaagt ggagaaggtt
     2461 ctttcaaaac agcacagctc ttctatcaat gcggtggcgt gggccccctc gggcttacat
     2521 gttgtcagtg tggacaaagg aagcagagct atgctgtggg cacagccttg aggtaccgcg
     2581 ggcccgggat ccaccggatc tagataactg atcataatca gccatac
//
