LOCUS       sequence-85379-e        2616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2616)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG16-N-18
FEATURES             Location/Qualifiers
     source          1..2616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1860..1869
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1866..2576
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2011..2029)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2279..2298
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct taccgccatg tcgtcgggcc
       61 tgcgcgccgc agactttccc cgctggaagc gtcacatcgc ggaggaactg aggcgccggg
      121 accgactgca gaggcaggcg ttcgaggaga tcattctgca gtataccaag ttgctggaaa
      181 agtcagatct tcattcagta ttgacccaga aactacaagc agaaaagcat gacatgccaa
      241 ataggcatga aataagtcct ggacatgatg gtgcgtggaa tgatagtcaa ctacaagaaa
      301 tggcccagtt gaggatcaaa caccaggaag agctgaccga actgcacaag aagcgtgggg
      361 agttagctca gttggtgatt gacctgaaca accaaatgca gcagaaggac aaggagatac
      421 agatgaatga agcaaagatt tcggagtatt tacagaccat ctctgacctg gagacaaact
      481 gcctggacct gcgcaccaaa ctgcaggacc tcgaggtagc caaccagacc ctgaaggatg
      541 agtatgacgc cctgcagatt acttttactg ccctagaaga gaaactgagg aaaactactg
      601 aggagaacca ggaactggtc accagatgga tggctgagaa ggcccaagaa gccaatcgcc
      661 tcaatgcaga gaatgagaag gactccagga ggcgtcaagc acggctgcag aaggagcttg
      721 cagaagcagc aaaggaacct ctacctgttg aacaggatga tgacattgaa gtcattgtgg
      781 atgagacctc agaccacaca gaagagacct ctcccgtccg agctgtcagc agagcagcta
      841 cgagacgctc tgtctcttcc atcccagtcc cccaggatat catggacact catcctgctt
      901 ctggtaaaga tgtgagagtc ccaactactg cctcgtatgt cttcgatgcg catgacggag
      961 aggtcaacgc agtgcagttc agtccaggct cccggttgct ggccactgga ggcatggacc
     1021 gcagggtgaa actttgggaa gcattcggag ataaatgtga attcaagggc tccctgtctg
     1081 gcagtaatgc tggaattaca agcattgaat ttgatagtgc tggagcttac ctattagcag
     1141 cttcaaatga ttttgcaagc cgaatctgga ctgtggatga ttatcgatta cggcacacac
     1201 tcacaggcca cagcgggaaa gtcctctctg ccaagttcct gctggacaat gcacggattg
     1261 tctcaggaag tcacgaccgg accctcaaac tctgggatct ccgcagcaaa gtctgcataa
     1321 aaacagtgtt tgcaggatcc agctgcaatg acattgtttg cactgaacaa tgtgtaatga
     1381 gtggacattt tgacaagaaa attcgtttct gggatatccg gtcagagagt gtggtccgag
     1441 agatggaact gttagggaag atcactgctc tggacctaaa ccctgagaga actgagctcc
     1501 tgagctgctc ccgtgatgac ctgctaaaag tcatcgacct ccggacaaat gcagtcaaac
     1561 agacattcag tgcgcctgga ttcaaatgcg gctctgactg gacccgggtt gtcttcagcc
     1621 ctgatggcag ttacgtggca gcaggctcag ccgagggttc tctttatgtc tggagtgtgc
     1681 tgacagggaa agtggagaag gttctttcaa aacagcacag ctcttctatc aatgcggtgg
     1741 cgtgggcccc ctcgggctta catgttgtca gtgtggacaa aggaagcaga gctatgctgt
     1801 gggcacagcc tgggagaggc agcgggagtg gcagtcgcgg aggcagtggt ggaccggtcg
     1861 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct
     1921 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg
     1981 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc
     2041 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga
     2101 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg
     2161 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
     2221 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
     2281 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
     2341 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
     2401 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
     2461 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
     2521 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaaagcg
     2581 gccgcgactc tagatcataa tcagccatac cacatt
//
