LOCUS       sequence-85383-e        1675 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane Receptor, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1675)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ATG5-N-14
FEATURES             Location/Qualifiers
     source          1..1675
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      907..916
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             913..1623
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1058..1076)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1326..1345
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgaga ccgccatgac agatgacaaa
       61 gatgtgcttc gagatgtgtg gtttggacga attccaactt gtttcacgct atatcaggat
      121 gagataactg aaagggaagc agaaccatac tatttgcttt tgccaagagt aagttatttg
      181 acgttggtga ctgacaaagt gaaaaagcac tttcagaagg ttatgagaca agaagacatt
      241 agtgagatat ggtttgaata tgaaggcaca ccactgaaat ggcattatcc aattggtttg
      301 ctatttgatc ttcttgcatc aagttcagct cttccttgga acatcacagt acattttaag
      361 agttttccag aaaaagacct tctgcactgt ccatctaagg atgcaattga agctcatttt
      421 atgtcatgta tgaaagaagc tgatgcttta aaacataaaa gtcaagtaat caatgaaatg
      481 cagaaaaaag atcacaagca actctggatg ggattgcaaa atgacagatt tgaccagttt
      541 tgggccatca atcggaaact catggaatat cctgcagaag aaaatggatt tcgttatatc
      601 ccctttagaa tatatcagac aacgactgaa agacctttca ttcagaagct gtttcgtcct
      661 gtggctgcag atggacagtt gcacacacta ggagatctcc tcaaagaagt ttgtccttct
      721 gctattgatc ctgaagatgg ggaaaaaaag aatcaagtga tgattcatgg aattgagcca
      781 atgttggaaa cacctctgca gtggctgagt gaacatctga gctacccgga taattttctt
      841 catattagta tcatcccaca gccaacagat gggagtggca gtcgcggaca gagtggtgga
      901 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      961 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     1021 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     1081 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     1141 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     1201 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     1261 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1321 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1381 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1441 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1501 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1561 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1621 taaagcggcc gcgactctag atcataatca gccataccac attgtagaga aatgt
//
