LOCUS       sequence-85384-e        2036 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2036)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-AuroraA-C-7
FEATURES             Location/Qualifiers
     source          1..2036
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      40..49
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             46..753
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(191..209)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     459..478
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1675..1686
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 caaggcctag tgacgtcaga tccgctagcg ctaccggtcg ccaccatggt gagcaagggc
       61 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      121 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      181 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      241 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      301 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      361 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      421 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      481 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag
      541 atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc
      601 tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac
      661 atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc
      721 cactccaccg gcggcatgga cgagctgtac aagtccggac tcagatctcg agccatggac
      781 cgatctaaag aaaactgcat ttcaggacct gttaaggcta cagctccagt tggaggtcca
      841 aaacgtgttc tcgtgactca gcaatttcct tgtcagaatc cattacctgt aaatagtggc
      901 caggctcagc gggtcttgtg tccttcaaat tcttcccagc gcgttccttt gcaagcacaa
      961 aagcttgtct ccagtcacaa gccggttcag aatcagaagc agaagcaatt gcaggcaacc
     1021 agtgtacctc atcctgtctc caggccactg aataacaccc aaaagagcaa gcagcccctg
     1081 ccatcggcac ctgaaaataa tcctgaggag gaactggcat caaaacagaa aaatgaagaa
     1141 tcaaaaaaga ggcagtgggc tttggaagac tttgaaattg gtcgccctct gggtaaagga
     1201 aagtttggta atgtttattt ggcaagagaa aagcaaagca agtttattct ggctcttaaa
     1261 gtgttattta aagctcagct ggagaaagcc ggagtggagc atcagctcag aagagaagta
     1321 gaaatacagt cccaccttcg gcatcctaat attcttagac tgtatggtta tttccatgat
     1381 gctaccagag tctacctaat tctggaatat gcaccacttg gaacagttta tagagaactt
     1441 cagaaacttt caaagtttga tgagcagaga actgctactt atataacaga attggcaaat
     1501 gccctgtctt actgtcattc gaagagagtt attcatagag acattaagcc agagaactta
     1561 cttcttggat cagctggaga gcttaaaatt gcagattttg ggtggtcagt acatgctcca
     1621 tcttccagga ggaccactct ctgtggcacc ctggactacc tgccccctga aatgattgaa
     1681 ggtcggatgc atgatgagaa ggtggatctc tggagccttg gagttctttg ctatgaattt
     1741 ttagttggga agcctccttt tgaggcaaac acataccaag agacctacaa aagaatatca
     1801 cgggttgaat tcacattccc tgactttgta acagagggag ccagggacct catttcaaga
     1861 ctgttgaagc ataatcccag ccagaggcca atgctcagag aagtacttga acacccctgg
     1921 atcacagcaa attcatcaaa accatcaaat tgccaaaaca aagaatcagc tagcaaacag
     1981 tcttagggat ccaccggatc tagataactg atcataatca gccataccac attttg
//
