LOCUS       sequence-85387-e        1399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cilium, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1399)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-B9D2-N-18
FEATURES             Location/Qualifiers
     source          1..1399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      631..640
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             637..1347
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(782..800)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1050..1069
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccatttctag tgacgtcaga tccgctagcg ctaccggact cagatctcga ggccaccatg
       61 gctgaagtgc acgtgatcgg gcagatcata ggggccaccg gtttctcgga aagcagcctc
      121 ttttgcaagt ggggcatcca cacaggggcc gcatggaagc tcctctcagg tgtacgagaa
      181 ggccagacac aggtagacac accccagata ggggacatgg cttactggtc ccaccccatt
      241 gacctgcact tcgctactaa aggtctccaa ggttggcctc gactccatct ccaggtgtgg
      301 tcccaggaca gctttggccg ctgccagctt gctggctatg gcttttgcca tgtgcctagc
      361 agcccaggca ctcatcagct ggactgccct acatggaggc ccctgggcag ttggagggag
      421 cagctagcac gggctttcgt gggtggtggg ccacagctgc tgcacgcaga taccatctac
      481 agcggggctg accgctaccg cctgcacacg gccgcgggtg gcacagtgca ccttggtatc
      541 ggcctgctgc tgcgccactt cgatcgctat ggagtggagt gtgtgggaat tctgcagtcg
      601 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggaggat
      661 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      721 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      781 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      841 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      901 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      961 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
     1021 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
     1081 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
     1141 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
     1201 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
     1261 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
     1321 ggcggcatgg acgagctgta caagtaaagc ggccgcgact ctagatcata atcagccata
     1381 ccacatgtag aagttgccc
//
