LOCUS       sequence-85389-e        2428 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane/Vesicles, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mCherry-C-Src-7
FEATURES             Location/Qualifiers
     source          1..2428
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..99
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      1672..1681
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1678..2388
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1823..1841)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2091..2110
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt cgaattcatg
       61 gggagcagca agagcaagcc caaggacccc agccagcgcc ggcgcagcct ggagccaccc
      121 gacagcaccc accacggggg attcccagcc tcgcagaccc ccaacaagac agcagccccc
      181 gacacgcacc gcacccccag ccgctccttt gggaccgtgg ccaccgagcc caagctcttc
      241 gggggcttca acacttctga caccgtcacg tcgccgcagc gtgccggggc actggctggc
      301 ggcgtcacca ctttcgtggc tctctatgac tacgagtccc ggactgaaac ggacttgtcc
      361 ttcaagaaag gagaacgcct gcagattgtc aacaacacgg aaggtgactg gtggctggct
      421 cattccctca ctacaggaca gacgggctac atccccagta actatgtcgc gccctcagac
      481 tccatccagg ctgaagagtg gtactttggg aagatcactc gtcgggagtc cgagcggctg
      541 ctgctcaacc ccgaaaaccc ccggggaacc ttcttggtcc gggagagcga gacgacaaaa
      601 ggtgcctatt gcctctccgt ttctgacttt gacaacgcca aggggctcaa tgtgaagcac
      661 tacaagatcc gcaagctgga cagcggcggc ttctacatca cctcacgcac acagttcagc
      721 agcctgcagc agctggtggc ctactactcc aaacatgctg atggcttgtg ccaccgcctg
      781 accaacgtct gccccacgtc caagccccag acccagggac tcgccaagga cgcgtgggaa
      841 atcccccggg agtcgctgcg gctggaggtg aagctggggc agggctgctt tggagaggtc
      901 tggatgggga cctggaacgg caccaccaga gtggccataa agactctgaa gcccggcacc
      961 atgtccccgg aggccttcct gcaggaagcc caagtgatga agaagctccg gcatgagaag
     1021 ctggttcagc tgtacgcagt ggtgtcggaa gagcccatct acatcgtcac tgagtacatg
     1081 agcaagggga gcctcctgga tttcctgaag ggagagatgg gcaagtacct gcggctgcca
     1141 cagctcgtcg atatggctgc tcagattgca tccggcatgg cctatgtgga gaggatgaac
     1201 tacgtgcacc gagacctgcg ggcggccaac atcctggtgg gggagaacct ggtgtgcaag
     1261 gtggctgact ttgggctggc acgcctcatc gaggacaacg agtacacagc acggcaaggt
     1321 gccaagttcc ccatcaagtg gacagccccc gaggcagccc tctatggccg gttcaccatc
     1381 aagtcggatg tctggtcctt cggcatcctg ctgactgagc tgaccaccaa gggccgggtg
     1441 ccatacccag ggatggtcaa cagggaggtg ctggaccagg tggagagggg ctaccgcatg
     1501 ccctgcccgc ccgagtgccc cgagtcgctg catgacctca tgtgccagtg ctggcggaag
     1561 gaccctgagg agcggcccac ttttgagtac ctgcaggcct tcctggagga ctacttcacc
     1621 tcgacagagc cccagtacca gcctggagag aacctacggg atccaccggt cgccaccatg
     1681 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     1741 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     1801 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     1861 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     1921 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     1981 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     2041 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     2101 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     2161 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     2221 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     2281 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     2341 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtaaag cggccgcgac
     2401 tctagatcat aatcagccat accacatt
//
