LOCUS       sequence-85393-e        2075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2075)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Calreticulin-N-16
FEATURES             Location/Qualifiers
     source          1..2075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1328..1337
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1334..2044
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1479..1497)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1747..1766
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgaggc caccatgctg ctatccgtgc cgctgctgct
       61 cggcctcctc ggcctggccg tcgccgagcc tgccgtctac ttcaaggagc agtttctgga
      121 cggagacggg tggacttccc gctggatcga atccaaacac aagtcagatt ttggcaaatt
      181 cgttctcagt tccggcaagt tctacggtga cgaggagaaa gataaaggtt tgcagacaag
      241 ccaggatgca cgcttttatg ctctgtcggc cagtttcgag cctttcagca acaaaggcca
      301 gacgctggtg gtgcagttca cggtgaaaca tgagcagaac atcgactgtg ggggcggcta
      361 tgtgaagctg tttcctaata gtttggacca gacagacatg cacggagact cagaatacaa
      421 catcatgttt ggtcccgaca tctgtggccc tggcaccaag aaggttcatg tcatcttcaa
      481 ctacaagggc aagaacgtgc tgatcaacaa ggacatccgt tgcaaggatg atgagtttac
      541 acacctgtac acactgattg tgcggccaga caacacctat gaggtgaaga ttgacaacag
      601 ccaggtggag tccggctcct tggaagacga ttgggacttc ctgccaccca agaagataaa
      661 ggatcctgat gcttcaaaac cggaagactg ggatgagcgg gccaagatcg atgatcccac
      721 agactccaag cctgaggact gggacaagcc cgagcatatc cctgaccctg atgctaagaa
      781 gcccgaggac tgggatgaag agatggacgg agagtgggaa cccccagtga ttcagaaccc
      841 tgagtacaag ggtgagtgga agccccggca gatcgacaac ccagattaca agggcacttg
      901 gatccaccca gaaattgaca accccgagta ttctcccgat cccagtatct atgcctatga
      961 taactttggc gtgctgggcc tggacctctg gcaggtcaag tctggcacca tctttgacaa
     1021 cttcctcatc accaacgatg aggcatacgc tgaggagttt ggcaacgaga cgtggggcgt
     1081 aacaaaggca gcagagaaac aaatgaagga caaacaggac gaggagcaga ggcttaagga
     1141 ggaggaagaa gacaagaaac gcaaagagga ggaggaggca gaggacaagg aggatgatga
     1201 ggacaaagat gaggatgagg aggatgagga ggacaaggag gaagatgagg aggaagatgt
     1261 ccccggccag gccaaggacg agctggcatc cgggggtgcg tcaggcggca gcggatctgg
     1321 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt
     1381 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1441 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     1501 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc
     1561 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
     1621 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
     1681 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1741 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1801 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg
     1861 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     1921 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt
     1981 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     2041 gtaaagcggc cgcgactcta gatcataatc agcca
//
