LOCUS       sequence-85395-e        1383 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1383)
  AUTHORS   Hanson CA, Drake KR, Baird MA, Han B, Kraft LJ, Davidson MW, 
            Kenworthy AK
  TITLE     Overexpression of caveolin-1 is sufficient to phenocopy the behavior
            of a disease-associated mutant.
  JOURNAL   Traffic. 2013 Jun;14(6):663-77. doi: 10.1111/tra.12066. Epub 2013 
            Apr 7.
  PUBMED    23469926
REFERENCE   2  (bases 1 to 1383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tra.12066";
            journalName: "Traffic"; date: "2013-06"; volume: "14"; issue: "6"; 
            pages: "663-77"
COMMENT     Sequence Label: mCherry-Caveolin-C-10
FEATURES             Location/Qualifiers
     source          1..1383
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctggca gcagtggagg ttctgggggc
      781 aaatacgtag actcggaggg acatctctac accgttccca tccgggaaca gggcaacatc
      841 tacaagccca acaacaaggc catggcagac gagctgagcg agaagcaagt gtacgacgcg
      901 cacaccaagg agatcgacct ggtcaaccgc gaccctaaac acctcaacga tgacgtggtc
      961 aagattgact ttgaagatgt gattgcagaa ccagaaggga cacacagttt tgacggcatt
     1021 tggaaggcca gcttcaccac cttcactgtg acgaaatact ggttttaccg cttgctgtct
     1081 gccctctttg gcatcccgat ggcactcatc tggggcattt acttcgccat tctctctttc
     1141 ctgcacatct gggcagttgt accatgcatt aagagcttcc tgattgagat tcagtgcatc
     1201 agccgtgtct attccatcta cgtccacacc gtctgtgacc cactctttga agctgttggg
     1261 aaaatattca gcaatgtccg catcaacttg cagaaagaaa tataagaatt ctgcagtcga
     1321 cggtaccgcg ggcccgggat ccaccggatc tagataactg atcataatca gccataccac
     1381 att
//
