LOCUS       sequence-85396-e        1674 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1674)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CCPB1-C-18
FEATURES             Location/Qualifiers
     source          1..1674
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..740
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(178..196)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     446..465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgtcagatcc gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggataaca
       61 tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca
      181 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt
      241 tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      361 tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg
      481 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc
      541 tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca
      661 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
      721 gcatggacga gctgtacaag tccggactca gatctcgaga gtccagtgga tattcgtctc
      781 ggtcgggtgg aagcagtgac cagcagctgg attgtgcctt ggatttgatg aggcgtctgc
      841 ctccgcagca gatagagaag aatctcagtg acctcattga cttggtccca agcctctgtg
      901 aagatctcct ctcctctgtt gatcagccat tgaagattgc acgagataag gtggtaggaa
      961 aagactatct attgtgtgac tacaacagag atggagactc gtatagatca ccgtggagta
     1021 acaagtatga tcctcccctg gaagacggtg ccatgccatc tgctcgcctg cgcaagctgg
     1081 aggtggaagc caacaatgcc tttgaccagt atagagactt gtatttcgaa ggtggagtct
     1141 cctctgtcta cctctgggat ctggatcatg gttttgccgg tgtgatcctc attaagaaag
     1201 ctggagatgg atcaaagaag attaagggat gctgggattc catccacgtg gtggaggttc
     1261 aggagaaatc cagcggccgt actgctcatt acaagctgac ctccacagtg atgctgtggc
     1321 tgcagactaa taaaactggt tctggtacca tgaaccttgg cgggagcctc accagacaga
     1381 tggagaaaga tgagactgtg agcgactctt ctccgcacat agccaatatt ggacgcttgg
     1441 tagaggacat ggaaaacaaa atcagaagta cactgaatga gatttatttt ggaaaaacaa
     1501 aggacatcgt gaatgggctg agatctattg atgctatccc cgacaaccaa aagtataagc
     1561 agttgcagag ggaactttct caagtgttga cccagcgcca gatctacatc cagcctgata
     1621 actaagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc taga
//
