LOCUS       sequence-85397-e        1660 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin Capping Protein , Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1660)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CCPB1-N-18
FEATURES             Location/Qualifiers
     source          1..1660
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      914..923
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             920..1630
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1065..1083)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1333..1352
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggactcag atctcgaggc caccatgagt gaccagcagc tggattgtgc
       61 cttggatttg atgaggcgtc tgcctccgca gcagatagag aagaatctca gtgacctcat
      121 tgacttggtc ccaagcctct gtgaagatct cctctcctct gttgatcagc cattgaagat
      181 tgcacgagat aaggtggtag gaaaagacta tctattgtgt gactacaaca gagatggaga
      241 ctcgtataga tcaccgtgga gtaacaagta tgatcctccc ctggaagacg gtgccatgcc
      301 atctgctcgc ctgcgcaagc tggaggtgga agccaacaat gcctttgacc agtatagaga
      361 cttgtatttc gaaggtggag tctcctctgt ctacctctgg gatctggatc atggttttgc
      421 cggtgtgatc ctcattaaga aagctggaga tggatcaaag aagattaagg gatgctggga
      481 ttccatccac gtggtggagg ttcaggagaa atccagcggc cgtactgctc attacaagct
      541 gacctccaca gtgatgctgt ggctgcagac taataaaact ggttctggta ccatgaacct
      601 tggcgggagc ctcaccagac agatggagaa agatgagact gtgagcgact cttctccgca
      661 catagccaat attggacgct tggtagagga catggaaaac aaaatcagaa gtacactgaa
      721 tgagatttat tttggaaaaa caaaggacat cgtgaatggg ctgagatcta ttgatgctat
      781 ccccgacaac caaaagtata agcagttgca gagggaactt tctcaagtgt tgacccagcg
      841 ccagatctac atccagcctg ataacgtggg aattctgcag tcgacggtac cgcgggcccg
      901 ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa
      961 ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat
     1021 cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac
     1081 caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc
     1141 caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga
     1201 gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca
     1261 ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt
     1321 cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg
     1381 gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga
     1441 cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct
     1501 gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac
     1561 catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct
     1621 gtacaagtaa agcggccgcg actctagatc ataatcagcc
//
