LOCUS       sequence-85398-e        2260 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2260)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..2260
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..734
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(172..190)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     440..459
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
       61 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      121 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      181 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      241 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      301 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      361 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      421 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
      481 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      541 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
      601 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
      661 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      721 acgagctgta caagtccgga ctcagatctg gcagcagtgg aggtggctgt gaccggaact
      781 gtgggctcat cgctggggct gtcattggtg ctgtcctggc tgtgtttgga ggtattctaa
      841 tgccagttgg agacctgctt atccagaaga caattaaaaa gcaagttgtc ctcgaagaag
      901 gtacaattgc ttttaaaaat tgggttaaaa caggcacaga agtttacaga cagttttgga
      961 tctttgatgt gcaaaatcca caggaagtga tgatgaacag cagcaacatt caagttaagc
     1021 aaagaggtcc ttatacgtac agagttcgtt ttctagccaa ggaaaatgta acccaggacg
     1081 ctgaggacaa cacagtctct ttcctgcagc ccaatggtgc catcttcgaa ccttcactat
     1141 cagttggaac agaggctgac aacttcacag ttctcaatct ggctgtggca gctgcatccc
     1201 atatctatca aaatcaattt gttcaaatga tcctcaattc acttattaac aagtcaaaat
     1261 cttctatgtt ccaagtcaga actttgagag aactgttatg gggctatagg gatccatttt
     1321 tgagtttggt tccgtaccct gttactacca cagttggtct gttttatcct tacaacaata
     1381 ctgcagatgg agtttataaa gttttcaatg gaaaagataa cataagtaaa gttgccataa
     1441 tcgacacata taaaggtaaa aggaatctgt cctattggga aagtcactgc gacatgatta
     1501 atggtacaga tgcagcctca tttccacctt ttgttgagaa aagccaggta ttgcagttct
     1561 tttcttctga tatttgcagg tcaatctatg ctgtatttga atccgacgtt aatctgaaag
     1621 gaatccctgt gtatagattt gttcttccat ccaaggcctt tgcctctcca gttgaaaacc
     1681 cagacaacta ttgtttctgc acagaaaaaa ttatctcaaa aaattgtaca tcatatggtg
     1741 tgctagacat cagcaaatgc aaagaaggga gacctgtgta catttcactt cctcattttc
     1801 tgtatgcaag tcctgatgtt tcagaaccta ttgatggatt aaacccaaat gaagaagaac
     1861 ataggacata cttggatatt gaacctataa ctggattcac tttacaattt gcaaaacggc
     1921 tgcaggtcaa cctattggtc aagccatcag aaaaaattca agtattaaag aatctgaaga
     1981 ggaactatat tgtgcctatt ctttggctta atgagactgg gaccattggt gatgagaagg
     2041 caaacatgtt cagaagtcaa gtaactggaa aaataaacct ccttggcctg atagaaatga
     2101 tcttactcag tgttggtgtg gtgatgtttg ttgcttttat gatttcatat tgtgcatgca
     2161 gatcgaaaac aataaaataa gaattctgca gtcgacggta ccgcgggccc gggatccacc
     2221 ggatctaaga taactgatca taatcagcca taccacattt
//
