LOCUS       sequence-85399-e        1597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1597)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CD81-10
FEATURES             Location/Qualifiers
     source          1..1597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(23..31,32..46)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      53..62
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             59..766
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(204..222)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     472..491
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tggtggacag caaatgggtc gggatctgta cgacgatgac gataaggatc ccgccaccat
       61 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
      121 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
      181 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
      241 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
      301 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
      361 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
      421 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
      481 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
      541 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
      601 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
      661 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
      721 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtccg gactcagatc
      781 tcgagctcaa gcttccggag tggagggctg caccaagtgc atcaagtacc tgctcttcgt
      841 cttcaatttc gtcttctggc tggctggagg cgtgatcctg ggtgtggccc tgtggctccg
      901 ccatgacccg cagaccacca acctcctgta tctggagctg ggagacaagc ccgcgcccaa
      961 caccttctat gtaggcatct acatcctcat cgctgtgggc gctgtcatga tgttcgttgg
     1021 cttcctgggc tgctacgggg ccatccagga atcccagtgc ctgctgggga cgttcttcac
     1081 ctgcctggtc atcctgtttg cctgtgaggt ggccgccggc atctggggct ttgtcaacaa
     1141 ggaccagatc gccaaggatg tgaagcagtt ctatgaccag gccctacagc aggccgtggt
     1201 ggatgatgac gccaacaacg ccaaggctgt ggtgaagacc ttccacgaga cgcttgactg
     1261 ctgtggctcc agcacactga ctgctttgac cacctcagtg ctcaagaaca atttgtgtcc
     1321 ctcgggcagc aacatcatca gcaacctctt caaggaggac tgccaccaga agatcgatga
     1381 cctcttctcc gggaagctgt acctcatcgg cattgctgcc atcgtggtcg ctgtgatcat
     1441 gatcttcgag atgatcctga gcatggtgct gtgctgtggc atccggaaca gctccgtgta
     1501 ctgaggcgga tccaagggcg aattccagca cactggcggc cgttactagt ggatccgagc
     1561 tcggtaccgc gggcccggga tccaccggat ctagata
//
