LOCUS       sequence-85400-e        1447 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Tetraspanin/Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1447)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CD9-10
FEATURES             Location/Qualifiers
     source          1..1447
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8..715
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(153..171)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     421..440
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgccaccatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg
       61 cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg
      121 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
      181 cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt
      241 gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg
      301 ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct
      361 gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg
      421 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga
      481 ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta
      541 cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta
      601 caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca
      661 gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccgg
      721 actcagatct cgagctcaag cttccccggt caaaggaggc accaagtgca tcaaatacct
      781 gctgttcgga tttaacttca tcttctggct tgccgggatt gctgtccttg ccattggact
      841 atggctccga ttcgactctc agaccaagag catcttcgag caagaaacta ataataataa
      901 ttccagcttc tacacaggag tctatattct gatcggagcc ggcgccctca tgatgctggt
      961 gggcttcctg ggctgctgcg gggctgtgca ggagtcccag tgcatgctgg gactgttctt
     1021 cggcttcctc ttggtgatat tcgccattga aatagctgcg gccatctggg gatattccca
     1081 caaggatgag gtgattaagg aagtccagga gttttacaag gacacctaca acaagctgaa
     1141 aaccaaggat gagccccagc gggaaacgct gaaagccatc cactatgcgt tgaactgctg
     1201 tggtttggct gggggcgtgg aacagtttat ctcagacatc tgccccaaga aggacgtact
     1261 cgaaaccttc accgtgaagt cctgtcctga tgccatcaaa gaggtcttcg acaataaatt
     1321 ccacatcatc ggcgcagtgg gcatcggcat tgccgtggtc atgatatttg gcatgatctt
     1381 cagtatgatc ttgtgctgtg ctatccgcag gaaccgcgag atggtctagg gatccaaggg
     1441 cgaattc
//
