LOCUS       sequence-85401-e        1395 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1395)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CDC42-C-10
FEATURES             Location/Qualifiers
     source          1..1395
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(180..198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             773..1348
                     /codon_start=1
                     /product="human Rho family small GTPase Cdc42"
                     /label=Cdc42
                     /translation="MQTIKCVVVGDGAVGKTCLLISYTTNKFPSEYVPTVFDNYAVTVM
                     IGGEPYTLGLFDTAGQEDYDRLRPLSYPQTDVFLVCFSVVSPSSFENVKEKWVPEITHH
                     CPKTPFLLVGTQIDLRDDPSTIEKLAKNKQKPITPETAEKLARDLKAVKYVECSALTQK
                     GLKNVFDEAILAALEPPEPKKSRRCVLL"
ORIGIN
        1 accgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      181 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      541 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctggt ggctccgcag gaatgcagac
      781 aattaagtgt gttgttgtgg gcgatggtgc tgttggtaaa acatgtctcc tgatatccta
      841 cacaacaaac aaatttccat cggaatatgt accgactgtt tttgacaact atgcagtcac
      901 agttatgatt ggtggagaac catatactct tggacttttt gatactgcag ggcaagagga
      961 ttatgacaga ttacgaccgc tgagttatcc acaaacagat gtatttctag tctgtttttc
     1021 agtggtctct ccatcttcat ttgaaaacgt gaaagaaaag tgggtgcctg agataactca
     1081 ccactgtcca aagactcctt tcttgcttgt tgggactcaa attgatctca gagatgaccc
     1141 ctctactatt gagaaacttg ccaagaacaa acagaagcct atcactccag agactgctga
     1201 aaagctggcc cgtgacctga aggctgtcaa gtatgtggag tgttctgcac ttacacagaa
     1261 aggcctaaag aatgtatttg acgaagcaat attggctgcc ctggagcctc cagaaccgaa
     1321 gaagagccgc aggtgtgtgc tgctatgagg atccaccgga tctagataac tgatcataat
     1381 cagccatacc acatt
//
