LOCUS       sequence-85402-e        1339 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1339)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1339)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1339
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    523..575
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      583..592
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             589..1299
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(734..752)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1002..1021
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gatccgctag ccaccatggg ccccaagcgg cggcagctga cgttccggga gaagtcgcgg
       61 atcatccagg aggtggagga gaacccggac ctacgcaagg gcgagatcgc gcggcgcttc
      121 aacatcccgc cgtccacgct gagcacgatc ctgaagaaca agcgcgccat cctggcgtcg
      181 gagcgcaagt acggggtggc ctccacctgc cgcaagacca acaagctgtc tccctacgac
      241 aagctcgagg gcttgctcat cgcctggttc cagcagatcc gcgccgccgg cctgccggtc
      301 aagggcatca tcctcaagga gaaggcgctg cgcatagccg aggagctggg catggacgac
      361 ttcaccgcct ccaacggctg gctggaccgc ttccgccggc gccacggcgt ggtgtcctgc
      421 agcggcgtgg cccgcgcccg cgcgcgaaac gctgcccccc gcaccccggc ggcgcctgcc
      481 agtccggccg cggtgccctc ggagggcagt ggcgggagca ctctcgagct caagcttcga
      541 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
      601 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
      661 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
      721 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
      781 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
      841 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
      901 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
      961 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     1021 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     1081 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     1141 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     1201 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     1261 cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     1321 taatcagcca taccacatt
//
