LOCUS       sequence-85405-e        1555 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1555)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1555
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      800..809
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             806..1516
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(951..969)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1219..1238
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gggacgtcag atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcgc
       61 ggccgcacgc tcctcgggac acagtgccaa ccatccagag gacaagatgg attggggcac
      121 actacagagc atcctcgggg gtgtcaacaa gcactccacc agcattggga aaatctggct
      181 cactgtcctc ttcatcttcc gcatcatgat cctcgtggtg gccgcgaagg aggtgtgggg
      241 agatgagcaa gccgattttg tttgcaacac tctccagcct ggctgtaaga atgtgtgcta
      301 cgaccactac ttccccatct ctcacatccg gctctgggct ctgcagctga tcatggtgtc
      361 cacgccggcc ctcctggtag ctatgcacgt ggcctaccgg agacacgaaa agaaacggaa
      421 gttcatgaag ggagagataa agaacgagtt taaggacatc gaagagatca aaacccagaa
      481 ggtccgtatc gaagggtccc tgtggtggac ctacaccacc agcatcttct tccgggtcat
      541 cttcgaagct gtcttcatgt atgtctttta catcatgtac aatggcttct tcatgcagcg
      601 tctggtgaag tgtaacgcct ggccttgtcc caatacagtg gactgcttca tttccaggcc
      661 cacagaaaag actgtcttca cggtgttcat gatctctgtg tctggaattt gcatcctgct
      721 aaacatcaca gagctgtgct atctgttcat taggtattgc tcagggaagt ccaaaagacc
      781 agtcgcggat ccaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat
      841 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
      901 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      961 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     1021 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     1081 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     1141 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     1201 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     1261 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     1321 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     1381 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     1441 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     1501 cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac cacat
//
