LOCUS       sequence-85406-e        1738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1738)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      983..992
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             989..1699
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1134..1152)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1402..1421
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattccctc
       61 tgggaaaggg cagcagcagc caggtgtggc agtgacaggg aggtgtgaat gaggcaggat
      121 gaactggaca ggtttgtaca ccttgctcag tggcgtgaac cggcattcta ctgccattgg
      181 ccgagtatgg ctctcggtca tcttcatctt cagaatcatg gtgctggtgg tggctgcaga
      241 gagtgtgtgg ggtgatgaga aatcttcctt catctgcaac acactccagc ctggctgcaa
      301 cagcgtttgc tatgaccaat tcttccccat ctcccatgtg cggctgtggt ccctgcagct
      361 catcctagtt tccaccccag ctctcctcgt ggccatgcac gtggctcacc agcaacacat
      421 agagaagaaa atgctacggc ttgagggcca tggggacccc ctacacctgg aggaggtgaa
      481 gaggcacaag gtccacatct cagggacact gtggtggacc tatgtcatca gcgtggtgtt
      541 ccggctgttg tttgaggccg tcttcatgta tgtcttttat ctgctctacc ctggctatgc
      601 catggtgcgg ctggtcaagt gcgacgtcta cccctgcccc aacacagtgg actgcttcgt
      661 gtcccgcccc accgagaaaa ccgtcttcac cgtcttcatg ctagctgcct ctggcatctg
      721 catcatcctc aatgtggccg aggtggtgta cctcatcatc cgggcctgtg cccgccgagc
      781 ccagcgccgc tccaatccac cttcccgcaa gggctcgggc ttcggccacc gcctctcacc
      841 tgaatacaag cagaatgaga tcaacaagct gctgagtgag caggatggct ccctgaaaga
      901 catactgcgc cgcagccctg gcaccggggc tgggctggct gaaaagagcg accgctgctc
      961 ggcctgcgcg gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc
     1021 catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga
     1081 gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct
     1141 gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat
     1201 gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc
     1261 cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac
     1321 cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg
     1381 caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc
     1441 ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa
     1501 gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc
     1561 cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga
     1621 ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat
     1681 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacat
//
