LOCUS       sequence-85407-e        2159 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2159)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Cx43-7
FEATURES             Location/Qualifiers
     source          1..2159
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1404..1413
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1410..2120
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1555..1573)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1823..1842
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcgctac cggactcaga tctcgagctc aagcttcgaa ttcggcagca
       61 cttttctttc attgggggga aggcgtgagg aaagtaccaa acagcagcag acttttaaac
      121 tttaaacaga caggtctgag agcctgaact ctcatttttc ctttgacttc agcctccaag
      181 gagttccacc aactttggcg cgccggcttc actttcatta agtgaaagag aggtgcccag
      241 acatgggtga ctggagtgcc ttggggaagc ttctggacaa ggtccaagcc tactccaccg
      301 ctggagggaa ggtgtggctg tcagtgctct tcatattcag aatcctgctc ctggggacag
      361 ctgttgagtc agcttggggt gatgaacagt ctgcctttcg ctgtaacact caacaacctg
      421 gctgcgaaaa cgtctgctat gacaagtcct tccccatctc tcacgtgcgc ttctgggtcc
      481 ttcagatcat attcgtgtct gtgcccacac tcctgtactt ggcccatgtg ttctatgtga
      541 tgaggaagga agagaagcta aacaagaaag aagaggagct caaagtggcc cagactgacg
      601 gggtcaacgt ggagatgcac ctgaagcaga ttgaaatcaa gaagttcaag tacgggattg
      661 aagagcacgg caaggtgaaa atgaggggcg gcttgctgag aacctacatc atcagcatcc
      721 tcttcaagtc tgtcttcgag gtggccttcc tgctcatcca gtggtacatc tatgggttca
      781 gcttgagcgc ggtctacacc tgcaagagag atccctgccc ccaccaggta gactgcttcc
      841 tctcacgtcc cacggagaaa accatcttca tcatcttcat gctggtggtg tccttggtgt
      901 ctctcgcttt gaacatcatt gagctcttct acgtcttctt caagggcgtt aaggatcgcg
      961 tgaagggaag aagcgatcct taccacgcca ccactggccc actgagccca tcaaaagact
     1021 gcggatctcc aaaatacgcc tacttcaatg gctgctcctc accaacggct ccactctcgc
     1081 ctatgtctcc tcctgggtac aagctggtta ctggtgacag aaacaattcc tcgtgccgca
     1141 attacaacaa gcaagctagc gagcaaaact gggcgaacta cagcgcagag caaaatcgca
     1201 tggggcaggc cggaagcacc atctccaact cgcacgccca gccgttcgat ttccccgacg
     1261 acaaccagaa tgccaaaaaa gttgctgctg gacatgaact ccagccatta gccatcgtgg
     1321 accaacgacc ttccagcaga gccagcagcc gcgccagcag caggcctcgg cctgatgacc
     1381 tggagattgc ggatccaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
     1441 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1501 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1561 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1621 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1681 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     1741 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     1801 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     1861 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     1921 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     1981 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2041 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2101 tggacgagct gtacaagtaa agcggccgcg actctagatc ataatcagcc ataccacat
//
