LOCUS       sequence-85409-e        1562 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1562)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Dectin1A-C-10
FEATURES             Location/Qualifiers
     source          1..1562
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             19..726
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(164..182)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     432..451
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcgctaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
       61 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      121 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      181 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      241 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      301 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      361 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
      421 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
      481 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
      541 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      601 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      661 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      721 tacaagtccg gactcagatc tggcagcagt ggaggtgaat atcatcctga tttagaaaat
      781 ttggatgaag atggatatac tcaattacac ttcgactctc aaagcaatac caggatagct
      841 gttgtttcag agaaaggatc gtgtgctgca tctcctcctt ggcgcctcat tgctgtaatt
      901 ttgggaatcc tatgcttggt aatactggtg atagctgtgg tcctgggtac catggctatt
      961 tggagatcca attcaggaag caacacattg gagaatggct actttctatc aagaaataaa
     1021 gagaaccaca gtcaacccac acaatcatct ttagaagaca gtgtgactcc taccaaagct
     1081 gtcaaaacca caggggttct ttccagccct tgtcctccta attggattat atatgagaag
     1141 agctgttatc tattcagcat gtcactaaat tcctgggatg gaagtaaaag acaatgctgg
     1201 caactgggct ctaatctcct aaagatagac agctcaaatg aattgggatt tatagtaaaa
     1261 caagtgtctt cccaacctga taattcattt tggataggcc tttctcggcc ccagactgaa
     1321 gtaccatggc tctgggagga tggatcaaca ttctcttcta acttatttca gatcagaacc
     1381 acagctaccc aagaaaaccc atctccaaat tgtgtatgga ttcacgtgtc agtcatttat
     1441 gaccaactgt gtagtgtgcc ctcatatagt atttgtgaga agaagttttc aatgtaagaa
     1501 ttctgcagtc gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat
     1561 tc
//
