LOCUS       sequence-85411-e        3509 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cadherin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3509)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3509)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmocollin1-C-18
FEATURES             Location/Qualifiers
     source          1..3509
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             23..730
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(168..186)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     436..455
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcta ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat
       61 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      121 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      181 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      241 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      301 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      361 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      421 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      481 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      541 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      601 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      661 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      721 gctgtacaag tccggactca gatctagtag cggaggtagc gcatccggcg gaagcggagg
      781 aagcgctctg gcctctgctg ccccagggag catcttctgt aagcagctcc ttttctctct
      841 cctggtttta acattacttt gcgatgcttg tcagaaagtt tatcttcgag ttccttctca
      901 tcttcaggct gaaacacttg taggcaaagt gaatctggag gagtgtctca agtcggccag
      961 cctaatccgg tccagtgacc ctgccttcag gattctagaa gatggctcaa tttacacaac
     1021 acatgacctc attttgtctt ctgaaaggaa aagtttttcc attttccttt cagatggtca
     1081 gagacgggaa caacaagaga taaaagttgt actgtcagca agagaaaaca agtctcctaa
     1141 gaagagacat accaaagaca cagccctcaa gcgcagcaag agacgatggg ctcctattcc
     1201 agcttcattg atggagaact cgttgggtcc atttccacaa cacgttcagc agatccaatc
     1261 tgatgctgca cagaattaca ccatctttta ttccataagt gggccaggcg tggacaaaga
     1321 acccttcaat ttgttttaca tagagaaaga cactggggat atcttttgta caaggagcat
     1381 tgaccgtgag aaatacgaac agtttgcgtt atatggctat gcaacaactg cagatggcta
     1441 tgcaccagaa tatccactcc ctttgatcat caaaattgaa gatgataatg ataacgcccc
     1501 atattttgaa cacagagtga ctatctttac tgtgcctgaa aattgccgat ccggcacttc
     1561 agtgggaaaa gtgaccgcca cagaccttga cgaacctgac actctccata ctcgtctgaa
     1621 atataaaatc ttacaacaaa tcccagatca tccaaagcat ttctccatac acccagatac
     1681 cggagtcatc accacaacta caccttttct ggatagagaa aaatgtgata cttaccagtt
     1741 aataatggaa gtgcgagaca tgggtggtca gcctttcggt ttatttaata caggaacaat
     1801 tactatttca cttgaggatg aaaatgacaa tccaccatct ttcacagaaa cttcttatgt
     1861 tacagaagta gaagaaaaca gaattgacgt ggagattttg cgaatgaagg tacaggatca
     1921 ggatttgcca aacactcctc actcaaaggc tgtatacaaa atcttacaag gaaatgaaaa
     1981 tggaaacttc ataattagca cagatccaaa tacaaatgaa ggagtgctgt gtgttgtcaa
     2041 gccattgaac tatgaagtca atcgccaagt tattttgcaa gttggtgtca ttaacgaggc
     2101 acaattctct aaagcagcga gctcacaaac tcctacaatg tgcactacaa ctgtcaccgt
     2161 taaaattata gacagtgatg agggccctga atgccaccct ccagtgaaag ttattcagag
     2221 tcaagatggc ttcccagctg gccaagaact ccttggatac aaagcactgg acccggaaat
     2281 atccagtggt gaaggcttaa ggtatcagaa gttaggggat gaagataact ggtttgaaat
     2341 taatcaacac actggcgact tgagaactct aaaagtacta gatagagaat ccaaatttgt
     2401 aaaaaacaac caatacaata tttcagttgt tgcagtggat gcagttggcc gatcttgcac
     2461 tggaacatta gtagttcatt tggatgatta caacgatcac gcacctcaaa ttgacaaaga
     2521 agtgaccatt tgtcagaata atgaggattt tgctgttctg aaacctgtag atccagatgg
     2581 acctgaaaat ggaccacctt ttcaattctt tctggataat tctgccagta aaaactggaa
     2641 catagaagaa aaggatggta aaactgccat tcttcgtcaa cggcaaaatc ttgattataa
     2701 ctattattct gtgcctattc aaataaaaga caggcatggt ttagttgcaa cacatatgtt
     2761 aacagtgaga gtatgtgact gttcaactcc atctgagtgt agaatgaagg ataaaagtac
     2821 aagagacgtt agaccaaatg taatacttgg aagatgggct attcttgcta tggtgttggg
     2881 ttctgtattg ttattatgta ttctgtttac atgtttctgt gtcactgcta agagaacagt
     2941 caagaaatgt tttccagaag acatagccca gcaaaattta attgtatcaa atactgaagg
     3001 acctggagaa gaagtaacgg aagcaaatat tagactcccc atgcagacat ccaacatttg
     3061 tgacacaagc atgtctgttg gtactgttgg tggccaggga atcaaaacac agcaaagttt
     3121 tgagatggtc aaaggaggct acactttgga ttccaacaaa ggaggtggac atcagacctt
     3181 ggagtccgtc aagggagtgg ggcagggaga tactggcaga tatgcgtaca cggactggca
     3241 gagtttcacc caacctcggc ttggcgaaaa ggtgtatttg tgtggacaag atgaggagca
     3301 taaacattgt gaagactacg tttgttcgta taactatgaa ggcaaaggtt ctctggccgg
     3361 ctcagtaggt tgctgcagcg atcggcagga agaagaggga ctggagtttc tagatcacct
     3421 ggaacccaaa tttaggacat tagcaaagac atgcatcaag aaataaggat ccaccggatc
     3481 tagataactg atcataatca gccatacca
//
