LOCUS       sequence-85412-e        3496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cadherin, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3496)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmocollin1-N-18
FEATURES             Location/Qualifiers
     source          1..3496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      7..16
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2743..2752
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2749..3459
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2894..2912)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3162..3181
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gctagcgcca ccatggctct ggcctctgct gccccaggga gcatcttctg taagcagctc
       61 cttttctctc tcctggtttt aacattactt tgcgatgctt gtcagaaagt ttatcttcga
      121 gttccttctc atcttcaggc tgaaacactt gtaggcaaag tgaatctgga ggagtgtctc
      181 aagtcggcca gcctaatccg gtccagtgac cctgccttca ggattctaga agatggctca
      241 atttacacaa cacatgacct cattttgtct tctgaaagga aaagtttttc cattttcctt
      301 tcagatggtc agagacggga acaacaagag ataaaagttg tactgtcagc aagagaaaac
      361 aagtctccta agaagagaca taccaaagac acagccctca agcgcagcaa gagacgatgg
      421 gctcctattc cagcttcatt gatggagaac tcgttgggtc catttccaca acacgttcag
      481 cagatccaat ctgatgctgc acagaattac accatctttt attccataag tgggccaggc
      541 gtggacaaag aacccttcaa tttgttttac atagagaaag acactgggga tatcttttgt
      601 acaaggagca ttgaccgtga gaaatacgaa cagtttgcgt tatatggcta tgcaacaact
      661 gcagatggct atgcaccaga atatccactc cctttgatca tcaaaattga agatgataat
      721 gataacgccc catattttga acacagagtg actatcttta ctgtgcctga aaattgccga
      781 tccggcactt cagtgggaaa agtgaccgcc acagaccttg acgaacctga cactctccat
      841 actcgtctga aatataaaat cttacaacaa atcccagatc atccaaagca tttctccata
      901 cacccagata ccggagtcat caccacaact acaccttttc tggatagaga aaaatgtgat
      961 acttaccagt taataatgga agtgcgagac atgggtggtc agcctttcgg tttatttaat
     1021 acaggaacaa ttactatttc acttgaggat gaaaatgaca atccaccatc tttcacagaa
     1081 acttcttatg ttacagaagt agaagaaaac agaattgacg tggagatttt gcgaatgaag
     1141 gtacaggatc aggatttgcc aaacactcct cactcaaagg ctgtatacaa aatcttacaa
     1201 ggaaatgaaa atggaaactt cataattagc acagatccaa atacaaatga aggagtgctg
     1261 tgtgttgtca agccattgaa ctatgaagtc aatcgccaag ttattttgca agttggtgtc
     1321 attaacgagg cacaattctc taaagcagcg agctcacaaa ctcctacaat gtgcactaca
     1381 actgtcaccg ttaaaattat agacagtgat gagggccctg aatgccaccc tccagtgaaa
     1441 gttattcaga gtcaagatgg cttcccagct ggccaagaac tccttggata caaagcactg
     1501 gacccggaaa tatccagtgg tgaaggctta aggtatcaga agttagggga tgaagataac
     1561 tggtttgaaa ttaatcaaca cactggcgac ttgagaactc taaaagtact agatagagaa
     1621 tccaaatttg taaaaaacaa ccaatacaat atttcagttg ttgcagtgga tgcagttggc
     1681 cgatcttgca ctggaacatt agtagttcat ttggatgatt acaacgatca cgcacctcaa
     1741 attgacaaag aagtgaccat ttgtcagaat aatgaggatt ttgctgttct gaaacctgta
     1801 gatccagatg gacctgaaaa tggaccacct tttcaattct ttctggataa ttctgccagt
     1861 aaaaactgga acatagaaga aaaggatggt aaaactgcca ttcttcgtca acggcaaaat
     1921 cttgattata actattattc tgtgcctatt caaataaaag acaggcatgg tttagttgca
     1981 acacatatgt taacagtgag agtatgtgac tgttcaactc catctgagtg tagaatgaag
     2041 gataaaagta caagagacgt tagaccaaat gtaatacttg gaagatgggc tattcttgct
     2101 atggtgttgg gttctgtatt gttattatgt attctgttta catgtttctg tgtcactgct
     2161 aagagaacag tcaagaaatg ttttccagaa gacatagccc agcaaaattt aattgtatca
     2221 aatactgaag gacctggaga agaagtaacg gaagcaaata ttagactccc catgcagaca
     2281 tccaacattt gtgacacaag catgtctgtt ggtactgttg gtggccaggg aatcaaaaca
     2341 cagcaaagtt ttgagatggt caaaggaggc tacactttgg attccaacaa aggaggtgga
     2401 catcagacct tggagtccgt caagggagtg gggcagggag atactggcag atatgcgtac
     2461 acggactggc agagtttcac ccaacctcgg cttggcgaaa aggtgtattt gtgtggacaa
     2521 gatgaggagc ataaacattg tgaagactac gtttgttcgt ataactatga aggcaaaggt
     2581 tctctggccg gctcagtagg ttgctgcagc gatcggcagg aagaagaggg actggagttt
     2641 ctagatcacc tggaacccaa atttaggaca ttagcaaaga catgcatcaa gaaaagcagc
     2701 ggtggagcaa gcgcagccag tggtagcgcg gatccaccgg tcgccaccat ggtgagcaag
     2761 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag
     2821 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     2881 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     2941 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc
     3001 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc
     3061 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     3121 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag
     3181 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc
     3241 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc
     3301 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg
     3361 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     3421 cgccactcca ccggcggcat ggacgagctg tacaagtaaa gcggccgcga ctctagatca
     3481 taatcagcca taccac
//
