LOCUS       sequence-85413-e        3976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3976)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmoglein1-C-18
FEATURES             Location/Qualifiers
     source          1..3976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..737
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(175..193)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     443..462
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagcaa gggcgaggag gataacatgg
       61 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      121 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      181 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      241 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      301 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      361 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      421 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      481 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
      541 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
      601 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
      661 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
      721 tggacgagct gtacaagtcc ggactcagat ctggcagcgg tggaggcagc gcatccggcg
      781 gaagcggaag cgactggagt ttcttcagag tagttgcagt gctgttcatt tttctggtgg
      841 tggtagaagt taacagtgag ttccgaatcc aggtaagaga ttataacact aaaaatggca
      901 ccatcaaatg gcattcaatc cgaaggcaga aacgtgaatg gatcaagttc gcagcagcct
      961 gtcgtgaagg tgaagacaac tcaaagagga acccaatcgc caaaattcac tcagattgtg
     1021 ctgcaaacca gcaagttaca taccgcatct ctggagtagg aattgatcag ccaccatatg
     1081 ggatctttgt cattaatcag aaaactggtg aaattaatat aacatccata gttgatcgag
     1141 aggtcactcc tttcttcatt atctactgcc gagctctgaa ctcaatgggc caagatttag
     1201 agaggcctct agagctcaga gtcagggttt tggatataaa tgacaaccct ccagtgtttt
     1261 caatggctac atttgcagga caaatagaag aaaattctaa tgcaaataca ctggtgatga
     1321 tactcaatgc tactgacgca gatgaaccga acaatttgaa ctcaaaaata gccttcaaga
     1381 ttataagaca agaaccttca gattcaccaa tgtttattat caacagaaat actggagaaa
     1441 ttcgaacgat gaataatttt ctagacagag agcaatacgg ccagtatgct cttgctgtaa
     1501 gaggctctga ccgagatggt ggggcagatg gcatgtcagc ggaatgtgag tgcaacatta
     1561 aaatcctcga tgtcaatgat aatatccctt acatggaaca gtcttcatat accatagaaa
     1621 ttcaagaaaa tactctaaat tcaaatttgc tcgaaattag agtaattgat ttggatgaag
     1681 agttctcagc taactggatg gcagtaattt tctttatctc tggaaatgaa ggaaattggt
     1741 ttgagataga aatgaatgaa agaacaaatg tgggaatttt aaaggttgtt aagcccttag
     1801 attatgaagc tatgcagagt ctgcaactca gtattggtgt cagaaataaa gctgaatttc
     1861 atcattcaat tatgtctcaa tataaactga aagcatctgc aatttctgtg actgtgttaa
     1921 atgtaattga aggcccagtg tttcgtccag gttcaaagac atatgttgta actggtaata
     1981 tgggatcaaa tgataaagtg ggagactttg tagctactga cctggacaca ggtagacctt
     2041 caacgactgt taggtatgta atgggaaata atccagctga cctgcttgct gttgattcaa
     2101 gaacaggcaa actcactttg aaaaataaag ttaccaagga acagtacaat atgctcggag
     2161 gaaaatacca aggaacgatt ctctctatag atgataatct tcaaagaact tgcactggta
     2221 caattaatat taacattcaa agttttggta atgacgacag gactaataca gagccgaaca
     2281 ctaaaattac taccaatact ggcagacaag aaagtacttc ttccactaac tatgatacca
     2341 gcacaacttc tactgactct agccaagtat attcttctga acccggaaac ggagccaaag
     2401 atttgttatc agacaatgta cattttggtc ctgctggcat tggactcctc atcatgggat
     2461 tcttggtctt aggattggtc ccatttttga tgatctgttg tgattgtgga ggtgctcctc
     2521 gtagtgcagc tggctttgag cctgttcccg aatgttcaga tggagcaatt cattcatggg
     2581 cagtagaagg accacagcct gaacccaggg atataaccac tgtcatacca caaataccac
     2641 ctgataacgc aaatataatt gaatgcattg acaactcagg agtttataca aatgagtatg
     2701 gtggcagaga aatgcaagac ctgggaggag gagagagaat gacaggattt gaactaacag
     2761 agggagttaa aacttcagga atgcctgaga tatgtcaaga atactctgga acattaagaa
     2821 gaaattctat gagggaatgt agagaaggag gtctgaatat gaatttcatg gaaagctact
     2881 tctgtcagaa agcatatgct tacgcagatg aagatgaagg acgcccatct aatgactgtt
     2941 tgctcatata tgacatcgaa ggtgtaggtt cccctgctgg ctctgtgggt tgttgtagct
     3001 tcattggaga agacctggat gacagcttct tggataccct gggacctaaa tttaagaagt
     3061 tggcagacat cagcctagga aaagaatcat atccagacct tgatccttct tggccaccac
     3121 aaagcactga accagtttgc cttcctcagg aaacagagcc cgttgttagt ggacacccac
     3181 caatctcccc acatttcggc actaccacag taatttctga gagcacctat ccctcgggac
     3241 ctggtgtact gcatcctaag cctattctcg atcctctggg ctatggtaat gtcactgtga
     3301 ccgagtctta caccacctct gacactctga agccctctgt gcacgttcac gataaccgac
     3361 cagcatcaaa cgtggtagtg acagagagag tggtcggccc aatctctggc gctgatttgc
     3421 atggaatgtt agagatgcct gacttgcgag atgggtcgaa tgttatagtg acagaaaggg
     3481 taatagcacc aagctctagt ctacccacct ctctgactat ccatcatcct agagagtctt
     3541 caaatgtggt agtgacagaa agagtaatcc aaccaacttc cggcatgata ggtagtctga
     3601 gtatgcaccc cgagttagcc aatgcccaca atgtcattgt gacagagagg gttgtttctg
     3661 gtgctggcgt aactggaatt agtggcacca ctgggatcag cggtggcata ggcagcagtg
     3721 gcctggttgg caccagcatg ggtgctggga gcggtgccct gagtggagct ggcataagtg
     3781 gtggtggcat tggcctgagc agcttgggag ggacagccag cattggccac atgaggagtt
     3841 cctctgacca tcactttaac caaaccattg ggtccgcctc ccctagcaca gctcgaagtc
     3901 gaatcacaaa gtatagtacc gtgcaatata gcaagtaggg atccaccgga tctagataac
     3961 tgatcataat cagcca
//
