LOCUS       sequence-85415-e        4171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4171)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 4171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmoglein2-C-18
FEATURES             Location/Qualifiers
     source          1..4171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      18..27
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             24..731
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(169..187)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     437..456
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca
       61 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
      121 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
      181 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
      241 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
      301 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
      361 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
      421 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
      481 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
      541 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
      601 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
      661 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
      721 agctgtacaa gtccggaagt ggagctggga gcggggcaag cggaagcgga agtggctccg
      781 gtggggcgcg gagcccggga cgcgcgtacg ccctgctgct tctcctgatc tgctttaacg
      841 ttggaagtgg acttcactta caggtcttaa gcacaagaaa tgaaaataag ctgcttccta
      901 aacatcctca tttagtgcgg caaaagcgcg cctggatcac cgcccccgtg gctcttcggg
      961 agggagagga tctgtccaag aagaatccaa ttgccaagat acattctgat cttgcagaag
     1021 aaagaggact caaaattact tacaaataca ctggaaaagg gattacagag ccaccttttg
     1081 gtatatttgt ctttaacaaa gatactggag aactgaatgt taccagcatt cttgatcgag
     1141 aagaaacacc attttttctg ctaacaggtt acgctttgga tgcaagagga aacaatgtag
     1201 agaaaccctt agagctacgc attaaggttc ttgatatcaa tgacaacgaa ccagtgttca
     1261 cacaggatgt ctttgttggg tctgttgaag agttgagtgc agcacatact cttgtgatga
     1321 aaatcaatgc aacagatgca gatgagccca ataccctgaa ctcgaaaatt tcctatagaa
     1381 tcgtatctct ggagcctgct tatcctccag tgttctacct aaataaagat acaggagaga
     1441 tttatacaac cagtgttacc ttggacagag aggaacacag cagctacact ttgacagtag
     1501 aagcaagaga tggcaatgga gaagttacag acaaacctgt aaaacaagct caagttcaga
     1561 ttcgtatttt ggatgtcaat gacaatatac ctgtagtaga aaataaagtg cttgaaggga
     1621 tggttgaaga aaatcaagtc aacgtagaag ttacgcgcat aaaagtgttc gatgcagatg
     1681 aaataggttc tgataattgg ctggcaaatt ttacatttgc atcaggaaat gaaggaggtt
     1741 atttccacat agaaacagat gctcaaacta acgaaggaat tgtgaccctt attaaggaag
     1801 tagattatga agaaatgaag aatcttgact tcagtgttat tgtcgctaat aaagcagctt
     1861 ttcacaagtc gattaggagt aaatacaagc ctacacccat tcccatcaag gtcaaagtga
     1921 aaaatgtgaa agaaggcatt cattttaaaa gcagcgtcat ctcaatttat gttagcgaga
     1981 gcatggatag atcaagcaaa ggccaaataa ttggaaattt tcaagcattt gatgaggaca
     2041 ctggactacc agcccatgca agatatgtaa aattagaaga tagagataat tggatctctg
     2101 tggattctgt cacatctgaa attaaacttg caaaacttcc tgattttgaa tctagatatg
     2161 ttcaaaatgg cacatacact gtaaagattg tggccatatc agaagattat cctagaaaaa
     2221 ccatcactgg cacagtcctt atcaatgttg aagacatcaa cgacaactgt cccacactga
     2281 tagagcctgt gcagacaatc tgtcacgatg cagagtatgt gaatgttact gcagaggacc
     2341 tggatggaca cccaaacagt ggccctttca gtttctccgt cattgacaaa ccacctggca
     2401 tggcagaaaa atggaaaata gcacgccaag aaagtaccag tgtgctgctg caacaaagtg
     2461 agaaaaagct agggagaagt gaaattcagt tcctgatttc agacaatcag ggttttagtt
     2521 gtcctgaaaa gcaggtcctt acactcacag tttgtgagtg tctgcatggc agcggctgca
     2581 gggaagcaca gcatgactcc tatgtgggcc tgggacccgc agcaattgcg ctcatgattt
     2641 tggcctttct gctcctgcta ttggtgccac ttttactgct gatgtgccat tgcggaaagg
     2701 gcgccaaagg ctttaccccc atacctggca ccatagagat gctgcatcct tggaataatg
     2761 aaggagcacc acctgaagac aaggtggtgc catcatttct gccagtggat caagggggca
     2821 gtctagtagg aagaaatgga gtaggaggta tggccaagga agccacgatg aaaggaagta
     2881 gctctgcttc cattgtcaaa gggcaacatg agatgtccga gatggatgga aggtgggaag
     2941 aacacagaag cctgctttct ggtagagcta cccagtttac aggggccaca ggcgctatca
     3001 tgaccactga aaccacgaag accgcaaggg ccacaggggc ttccagagac atggccggag
     3061 ctcaggcagc tgctgttgca ctgaacgaag agttcttaag aaattatttc actgataaag
     3121 cggcctctta cactgaggaa gatgaaaatc acacagccaa agattgcctt ctggtttatt
     3181 ctcaggaaga aactgaatcg ctgaatgctt ctattggttg ttgcagtttt attgaaggag
     3241 agctagatga ccgcttctta gatgatttgg gacttaaatt caagacacta gctgaagttt
     3301 gcctgggtca aaaaatagat ataaataagg aaattgagca gagacaaaaa cctgccacag
     3361 aaacaagtat gaacacagct tcacattcac tctgtgagca aactatggtt aattcagaga
     3421 atacctactc ctctggcagt agcttcccag ttccaaaatc tttgcaagaa gccaatgcag
     3481 agaaagtaac tcaggaaata gtcactgaaa ggtctgtgtc ttctaggcag gcgcaaaagg
     3541 tagctacacc tcttcctgac ccaatggctt ctagaaatgt gatagcaaca gaaacttcct
     3601 atgtcacagg gtccactatg ccaccaacca ctgtgatcct gggtcctagc cagccacaga
     3661 gccttattgt gacagagagg gtgtatgctc cagcttctac cttggtagat cagccttatg
     3721 ctaatgaagg tacagttgtg gtcactgaaa gagtaataca gcctcatggg ggtggatcga
     3781 atcctctgga aggcactcag catcttcaag atgtacctta cgtcatggtg agggaaagag
     3841 agagcttcct tgcccccagc tcaggtgtgc agcctactct ggccatgcct aatatagcag
     3901 taggacagaa tgtgacagtg acagaaagag ttctagcacc tgcttccact ctgcaatcca
     3961 gttaccagat tcccactgaa aattctatga cggctaggaa caccacggtg tctggagctg
     4021 gagtccctgg ccctctgcca gattttggtt tagaggaatc tggtcattct aattctacca
     4081 taaccacatc ttccaccaga gttaccaagc atagcactgt acagcattct tactcctaag
     4141 gatccaccgg atctagataa ctgatcataa t
//
