LOCUS       sequence-85416-e        4161 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Desmosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4161)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 4161)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Desmoglein2-N-18
FEATURES             Location/Qualifiers
     source          1..4161
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      3427..3436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3433..4143
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3578..3596)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3846..3865
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcagatccg ctagcgtcgc caccatggcg cggagcccgg gacgcgcgta cgccctgctg
       61 cttctcctga tctgctttaa cgttggaagt ggacttcact tacaggtctt aagcacaaga
      121 aatgaaaata agctgcttcc taaacatcct catttagtgc ggcaaaagcg cgcctggatc
      181 accgcccccg tggctcttcg ggagggagag gatctgtcca agaagaatcc aattgccaag
      241 atacattctg atcttgcaga agaaagagga ctcaaaatta cttacaaata cactggaaaa
      301 gggattacag agccaccttt tggtatattt gtctttaaca aagatactgg agaactgaat
      361 gttaccagca ttcttgatcg agaagaaaca ccattttttc tgctaacagg ttacgctttg
      421 gatgcaagag gaaacaatgt agagaaaccc ttagagctac gcattaaggt tcttgatatc
      481 aatgacaacg aaccagtgtt cacacaggat gtctttgttg ggtctgttga agagttgagt
      541 gcagcacata ctcttgtgat gaaaatcaat gcaacagatg cagatgagcc caataccctg
      601 aactcgaaaa tttcctatag aatcgtatct ctggagcctg cttatcctcc agtgttctac
      661 ctaaataaag atacaggaga gatttataca accagtgtta ccttggacag agaggaacac
      721 agcagctaca ctttgacagt agaagcaaga gatggcaatg gagaagttac agacaaacct
      781 gtaaaacaag ctcaagttca gattcgtatt ttggatgtca atgacaatat acctgtagta
      841 gaaaataaag tgcttgaagg gatggttgaa gaaaatcaag tcaacgtaga agttacgcgc
      901 ataaaagtgt tcgatgcaga tgaaataggt tctgataatt ggctggcaaa ttttacattt
      961 gcatcaggaa atgaaggagg ttatttccac atagaaacag atgctcaaac taacgaagga
     1021 attgtgaccc ttattaagga agtagattat gaagaaatga agaatcttga cttcagtgtt
     1081 attgtcgcta ataaagcagc ttttcacaag tcgattagga gtaaatacaa gcctacaccc
     1141 attcccatca aggtcaaagt gaaaaatgtg aaagaaggca ttcattttaa aagcagcgtc
     1201 atctcaattt atgttagcga gagcatggat agatcaagca aaggccaaat aattggaaat
     1261 tttcaagcat ttgatgagga cactggacta ccagcccatg caagatatgt aaaattagaa
     1321 gatagagata attggatctc tgtggattct gtcacatctg aaattaaact tgcaaaactt
     1381 cctgattttg aatctagata tgttcaaaat ggcacataca ctgtaaagat tgtggccata
     1441 tcagaagatt atcctagaaa aaccatcact ggcacagtcc ttatcaatgt tgaagacatc
     1501 aacgacaact gtcccacact gatagagcct gtgcagacaa tctgtcacga tgcagagtat
     1561 gtgaatgtta ctgcagagga cctggatgga cacccaaaca gtggcccttt cagtttctcc
     1621 gtcattgaca aaccacctgg catggcagaa aaatggaaaa tagcacgcca agaaagtacc
     1681 agtgtgctgc tgcaacaaag tgagaaaaag ctagggagaa gtgaaattca gttcctgatt
     1741 tcagacaatc agggttttag ttgtcctgaa aagcaggtcc ttacactcac agtttgtgag
     1801 tgtctgcatg gcagcggctg cagggaagca cagcatgact cctatgtggg cctgggaccc
     1861 gcagcaattg cgctcatgat tttggccttt ctgctcctgc tattggtgcc acttttactg
     1921 ctgatgtgcc attgcggaaa gggcgccaaa ggctttaccc ccatacctgg caccatagag
     1981 atgctgcatc cttggaataa tgaaggagca ccacctgaag acaaggtggt gccatcattt
     2041 ctgccagtgg atcaaggggg cagtctagta ggaagaaatg gagtaggagg tatggccaag
     2101 gaagccacga tgaaaggaag tagctctgct tccattgtca aagggcaaca tgagatgtcc
     2161 gagatggatg gaaggtggga agaacacaga agcctgcttt ctggtagagc tacccagttt
     2221 acaggggcca caggcgctat catgaccact gaaaccacga agaccgcaag ggccacaggg
     2281 gcttccagag acatggccgg agctcaggca gctgctgttg cactgaacga agagttctta
     2341 agaaattatt tcactgataa agcggcctct tacactgagg aagatgaaaa tcacacagcc
     2401 aaagattgcc ttctggttta ttctcaggaa gaaactgaat cgctgaatgc ttctattggt
     2461 tgttgcagtt ttattgaagg agagctagat gaccgcttct tagatgattt gggacttaaa
     2521 ttcaagacac tagctgaagt ttgcctgggt caaaaaatag atataaataa ggaaattgag
     2581 cagagacaaa aacctgccac agaaacaagt atgaacacag cttcacattc actctgtgag
     2641 caaactatgg ttaattcaga gaatacctac tcctctggca gtagcttccc agttccaaaa
     2701 tctttgcaag aagccaatgc agagaaagta actcaggaaa tagtcactga aaggtctgtg
     2761 tcttctaggc aggcgcaaaa ggtagctaca cctcttcctg acccaatggc ttctagaaat
     2821 gtgatagcaa cagaaacttc ctatgtcaca gggtccacta tgccaccaac cactgtgatc
     2881 ctgggtccta gccagccaca gagccttatt gtgacagaga gggtgtatgc tccagcttct
     2941 accttggtag atcagcctta tgctaatgaa ggtacagttg tggtcactga aagagtaata
     3001 cagcctcatg ggggtggatc gaatcctctg gaaggcactc agcatcttca agatgtacct
     3061 tacgtcatgg tgagggaaag agagagcttc cttgccccca gctcaggtgt gcagcctact
     3121 ctggccatgc ctaatatagc agtaggacag aatgtgacag tgacagaaag agttctagca
     3181 cctgcttcca ctctgcaatc cagttaccag attcccactg aaaattctat gacggctagg
     3241 aacaccacgg tgtctggagc tggagtccct ggccctctgc cagattttgg tttagaggaa
     3301 tctggtcatt ctaattctac cataaccaca tcttccacca gagttaccaa gcatagcact
     3361 gtacagcatt cttactccag cagcggtgga gcaagcgcag ccagtggtag cgcggatcca
     3421 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
     3481 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
     3541 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
     3601 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
     3661 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
     3721 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
     3781 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     3841 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     3901 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     3961 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     4021 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     4081 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     4141 taaagcggcc gcgactctag a
//
