LOCUS       sequence-85420-e        1647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1647)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-EB1-C-18
FEATURES             Location/Qualifiers
     source          1..1647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(179..197)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     447..466
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      241 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      541 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctggca gcggtggagg cagcgcatcc
      781 ggcggaagcg gaagcgcagt gaacgtatac tcaacgtcag tgaccagtga taacctaagt
      841 cgacatgaca tgctggcctg gatcaatgag tctctgcagt tgaatctgac aaagatcgaa
      901 cagttgtgct caggggctgc gtattgtcag tttatggaca tgctgttccc tggctccatt
      961 gccttgaaga aagtgaaatt ccaagctaag ctagaacacg agtacatcca gaacttcaaa
     1021 atattacaag caggttttaa gagaatgggt gttgacaaaa taattcctgt ggacaaatta
     1081 gtaaaaggaa agtttcagga caattttgaa ttcgttcagt ggttcaagaa gttcttcgat
     1141 gcaaactatg atggaaaaga ctatgaccct gtggctgcta gacaaggtca agaaactgca
     1201 gtggctcctt cccttgttgc tccagctctg aataaaccga agaaacctct cacttctagc
     1261 agtgcagctc cccagaggcc catctcaaca cagagaaccg ctgcggctcc taaggctggc
     1321 cctggtgtgg tgcgaaagaa ccctggtgtg ggcaacggag acgacgaggc agctgagttg
     1381 atgcagcagg tcaacgtatt gaaacttact gttgaagact tggagaaaga gagggatttc
     1441 tacttcggaa agctacggaa cattgaattg atttgccagg agaacgaggg ggaaaacgac
     1501 cctgtattgc agaggattgt agacattctg tatgccacag atgaaggctt tgtgatacct
     1561 gatgaagggg gcccacagga ggagcaagaa gagtattaag gatccaccgg atctagataa
     1621 ctgatcataa tcagccatac cacattt
//
