LOCUS       sequence-85422-e        1694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1694)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-EB3-C-20
FEATURES             Location/Qualifiers
     source          1..1694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             37..744
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(182..200)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     450..469
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtgacgtcag atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat
       61 aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac
      121 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
      181 gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct
      241 cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg
      301 aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc
      361 gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag
      421 ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg
      481 gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag
      541 aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc
      601 aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc
      661 cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc
      721 ggcggcatgg acgagctgta caagtccgga ctcagatctg gcagcggtgg aggcagcgca
      781 tccggcggaa gcggaagcgg gggagccgtc aatgtgtact ccacatctgt gaccagtgaa
      841 aatctgagtc gccatgatat gcttgcatgg gtcaacgact ccctgcacct caactatacc
      901 aagatagaac agctttgttc aggggcagcc tactgccagt tcatggacat gctcttcccc
      961 ggctgtgtgc acttgaggaa agtgaagttc caggccaaac tagagcatga atacatccac
     1021 aacttcaagg tgctgcaagc agctttcaag aagatgggtg ttgacaaaat cattcctgta
     1081 gagaaattag tgaaaggaaa attccaagat aattttgagt ttattcagtg gtttaagaaa
     1141 ttctttgacg caaactatga tggaaaggat tacaaccctc tgctggcgcg gcagggccag
     1201 gacgtagcgc cacctcctaa cccaggtgat cagatattca acaaatccaa gaaactcatt
     1261 ggcacagcag ttccacagag gacgtccccc acaggcccaa aaaacatgca gacctctggc
     1321 cggctgagca atgtggcccc cccctgcatt ctccggaaga atcctccatc agcccgaaat
     1381 ggcggccatg agactgatgc ccaaattctt gaactcaacc aacagctggt ggacttgaag
     1441 ctgacagtgg atgggctgga gaaggaacgt gacttctact tcagcaaact tcgtgacatc
     1501 gagctcatct gccaggagca tgaaagtgaa aacagccctg ttatctcagg catcattggc
     1561 atcctctatg ccacagagga aggattcgca ccccctgagg acgatgagat tgaagagcat
     1621 caacaagaag accaggacga gtactaagga tccaccggat ctagataact gatcataatc
     1681 agccatacca catt
//
