LOCUS       sequence-85423-e        3403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytoplasm, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3403)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-EIF2C3-N-18
FEATURES             Location/Qualifiers
     source          1..3403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2653..2662
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2659..3369
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2804..2822)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3072..3091
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatggaa atcggctccg caggacccgc tggggcccag
       61 cccctactca tggtgcccag aagacctggc tatggcacca tgggcaaacc cattaaactg
      121 ctggctaact gttttcaagt tgaaatccca aagattgatg tctacctcta tgaggtagat
      181 attaaaccag acaagtgtcc taggagagtg aacagggagg tggttgactc aatggttcag
      241 cattttaaag taactatatt tggagaccgt agaccagttt atgatggaaa aagaagtctt
      301 tacaccgcca atccacttcc tgtggcaact acaggggtag atttagacgt tactttacct
      361 ggggaaggtg gaaaagatcg acctttcaag gtgtcaatca aatttgtctc tcgggtgagt
      421 tggcacctac tgcatgaagt actgacagga cggaccttgc ctgagccact ggaattagac
      481 aagccaatca gcactaaccc tgtccatgcc gttgatgtgg tgctacgaca tctgccctcc
      541 atgaaataca cacctgtggg gcgttcattt ttctccgctc cagaaggata tgaccaccct
      601 ctgggagggg gcagggaagt gtggtttgga ttccatcagt ctgttcggcc tgccatgtgg
      661 aaaatgatgc ttaatatcga tgtttctgcc actgccttct acaaagcaca acctgtaatt
      721 cagttcatgt gtgaagttct tgatattcat aatattgatg agcaaccaag acctctgact
      781 gattctcatc gggtaaaatt caccaaagag ataaaaggtt tgaaggttga agtgactcat
      841 tgtggaacaa tgagacggaa ataccgtgtt tgtaatgtaa caaggaggcc tgccagtcat
      901 caaacctttc ctttacagtt agaaaacggc caaactgtgg agagaacagt agcgcagtat
      961 ttcagagaaa agtatactct tcagctgaag tacccgcacc ttccctgtct gcaagtcggg
     1021 caggaacaga aacacaccta cctgccacta gaagtctgta atattgtggc agggcaacga
     1081 tgtatcaaga agctaacaga caatcagact tccactatga tcaaggcaac agcaagatct
     1141 gcaccagata gacaagagga aattagcaga ttggtaagaa gtgcaaatta tgaaacagat
     1201 ccatttgttc aggagtttca atttaaagtt cgggatgaaa tggctcatgt aactggacgc
     1261 gtacttccag cacctatgct ccagtatgga ggacggaatc ggacagtagc aacaccgagc
     1321 catggagtat gggacatgcg agggaaacaa ttccacacag gagttgaaat caaaatgtgg
     1381 gctatcgctt gttttgccac acagaggcag tgcagagaag aaatattgaa gggtttcaca
     1441 gaccagctgc gtaagatttc taaggatgca gggatgccca tccagggcca gccatgcttc
     1501 tgcaaatatg cacagggggc agacagcgta gagcccatgt tccggcatct caagaacaca
     1561 tattctggcc tacagcttat tatcgtcatc ctgccgggga agacaccagt gtatgcggaa
     1621 gtgaaacgtg taggagacac acttttgggt atggctacac aatgtgttca agtcaagaat
     1681 gtaataaaaa catctcctca aactctgtca aacttgtgcc taaagataaa tgttaaactc
     1741 ggagggatca ataatattct tgtacctcat caaagacctt ctgtgttcca gcaaccagtg
     1801 atctttttgg gagccgatgt cactcatcca cctgctggtg atggaaagaa gccttctatt
     1861 gctgctgttg taggtagtat ggatgcacac ccaagcagat actgtgccac agtaagagtt
     1921 cagagacccc gacaggagat catccaggac ttggcctcca tggtccggga acttcttatt
     1981 caattttata agtcaactcg gttcaagcct actcgtatca tcttttatcg ggatggtgtt
     2041 tcagaggggc agtttaggca ggtattatat tatgaactac tagcaattcg agaagcctgc
     2101 atcagtttgg agaaagacta tcaacctgga ataacctaca ttgtagttca gaagagacat
     2161 cacactcgat tattttgtgc tgataggaca gaaagggttg gaagaagtgg caatatccca
     2221 gctggaacaa cagttgatac agacattaca cacccatatg agttcgattt ttacctctgt
     2281 agccatgctg gaatacaggg taccagtcgt ccttcacact atcatgtttt atgggatgat
     2341 aactgcttta ctgcagatga acttcagctg ctaacttacc agctctgcca cacttacgta
     2401 cgctgtacac gatctgtttc tatacctgca ccagcgtatt atgctcacct ggtagcattt
     2461 agagccagat atcatcttgt ggacaaagaa catgacagtg ctgaaggaag tcacgtttca
     2521 ggacaaagca atgggcgaga tccacaagct cttgccaagg ctgtacagat tcaccaagat
     2581 accttacgca caatgtactt cgcttctcga attctgcagt cgacggtacc gcgggcccgg
     2641 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     2701 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     2761 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     2821 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     2881 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     2941 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     3001 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     3061 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     3121 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     3181 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     3241 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     3301 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     3361 tacaagtaaa gcggccgcga ctctagatca taatcagcca tac
//
