LOCUS       sequence-85424-e        1475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1475)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Endo-14
FEATURES             Location/Qualifiers
     source          1..1475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(180..198)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     448..467
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             788..817
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggaggataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
      181 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
      541 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggatc agatctcgag ctcaagcttg atatcgaatt
      781 ccccatggag cagaagctga tctccgagga ggacctgaac cggaagaaac tggtggttgt
      841 tggtgatgga gcctgtggaa agacatgctt gctcatagtc ttcagcaagg acgagttccc
      901 agaggtgtat gtgcccacag tgtttgagaa ctatgtggca gatatcgagg tggatggaaa
      961 gcaggtagag ttggctttgt gggacacagc tggccaggag gactacgacc gcctgcggcc
     1021 gctctcctac ccggacaccg acgtcattct catgtgcttc tcggtggaca gcccggactc
     1081 gctggagaac atccccgaga agtgggtccc cgaggtgaag cacttctgtc ccaatgtgcc
     1141 catcatcctg gtggccaaca aaaaagacct gcgcagcgac gagcatgtcc gcacagagct
     1201 ggcccgcatg aagcaggaac ccgttcgcac ggatgacggc cgcgccatgg ccgtgcgcat
     1261 ccaagcctac gactacctcg agtgctctgc caagaccaag gaaggcgtgc gcgaggtctt
     1321 cgagacggcc acgcgcgccg cgctgcagaa gcgctacggc tcccagaacg gctgcatcaa
     1381 ctgctgcaag gtgctatgag ggccgcgccc gtcgcgcctg cccctgccgg ggatccaccg
     1441 gatctagata actgatcata atcagccata ccaca
//
