LOCUS       sequence-85425-e        1091 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 587, Emission: 610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1091)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-ER-3
FEATURES             Location/Qualifiers
     source          1..1091
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        43..94
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     45..65
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             183..890
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(328..346)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     596..615
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
       61 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta
      121 gcatgctgct atccgtgccg ttgctgctcg gcctcctcgg cctggccgtc gccgaccggt
      181 cgatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
      241 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
      301 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
      361 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
      421 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
      481 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
      541 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
      601 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
      661 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
      721 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
      781 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
      841 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag aaggacgagc
      901 tgtaagaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggatc tagataactg
      961 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     1021 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     1081 gcttataatg g
//
