LOCUS       sequence-85426-e        2581 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 
            610.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2581)
  TITLE     Davidson lab - mCherry constructs
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mCherry-Ezrin-C-14
FEATURES             Location/Qualifiers
     source          1..2581
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      21..30
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             27..734
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(172..190)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     440..459
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atccgctagc gctaccggtc gccaccatgg tgagcaaggg cgaggaggat aacatggcca
       61 tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt
      121 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
      181 aggtgaccaa gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt
      241 acggctccaa ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct
      301 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
      361 tgacccagga ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca
      421 ccaacttccc ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct
      481 ccgagcggat gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc
      541 tgaaggacgg cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg
      601 tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg
      661 actacaccat cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg
      721 acgagctgta caagtccgga ctcagatctc gaggaagcgg cggatctggg tccggtccga
      781 aaccaatcaa tgtccgagtt accaccatgg atgcagagct ggagtttgca atccagccaa
      841 atacaactgg aaaacagctt tttgatcagg tggtaaagac tatcggcctc cgggaagtgt
      901 ggtactttgg cctccactat gtggataata aaggatttcc tacctggctg aagctggata
      961 agaaggtgtc tgcccaggag gtcaggaagg agaatcccct ccagttcaag ttccgggcca
     1021 agttctaccc tgaagatgtg gctgaggagc tcatccagga catcacccag aaacttttct
     1081 tcctccaagt gaaggaagga atccttagcg atgagatcta ctgcccccct gagactgccg
     1141 tgctcttggg gtcctacgct gtgcaggcca agtttgggga ctacaacaaa gaagtgcaca
     1201 agtctgggta cctcagctct gagcggctga tccctcaaag agtgatggac cagcacaaac
     1261 ttaccaggga ccagtgggag gaccggatcc aggtgtggca tgcggaacac cgtgggatgc
     1321 tcaaagataa tgctatgttg gaatacctga agattgctca ggacctggaa atgtatggaa
     1381 tcaactattt cgagataaaa aacaagaaag gaacagacct ttggcttgga gttgatgccc
     1441 ttggactgaa tatttatgag aaagatgata agttaacccc aaagattggc tttccttgga
     1501 gtgaaatcag gaacatctct ttcaatgaca aaaagtttgt cattaaaccc atcgacaaga
     1561 aggcacctga ctttgtgttt tatgccccac gtctgagaat caacaagcgg atcctgcagc
     1621 tctgcatggg caaccatgag ttgtatatgc gccgcaggaa gcctgacacc atcgaggtgc
     1681 agcagatgaa ggcccaggcc cgggaggaga agcatcagaa gcagctggag cggcaacagc
     1741 tggaaacaga gaagaaaagg agagaaaccg tggagagaga gaaagagcag atgatgcgcg
     1801 agaaggagga gttgatgctg cggctgcagg actatgagga gaagacaaag aaggcagaga
     1861 gagagctctc ggagcagatt cagagggccc tgcagctgga ggaggagagg aagcgggcac
     1921 aggaggaggc cgagcgccta gaggctgacc gtatggctgc actgcgggct aaggaggagc
     1981 tggagagaca ggcggtggat cagataaaga gccaggagca gctggctgcg gagcttgcag
     2041 aatacactgc caagattgcc ctcctggaag aggcgcggag gcgcaaggag gatgaagttg
     2101 aagagtggca gcacagggcc aaagaagccc aggatgacct ggtgaagacc aaggaggagc
     2161 tgcacctggt gatgacagca cccccgcccc caccaccccc cgtgtacgag ccggtgagct
     2221 accatgtcca ggagagcttg caggatgagg gcgcagagcc cacgggctac agcgcggagc
     2281 tgtctagtga gggcatccgg gatgaccgca atgaggagaa gcgcatcact gaggcagaga
     2341 agaacgagcg tgtgcagcgg cagctgctga cgctgagcag cgagctgtcc caggcccgag
     2401 atgagaataa gaggacccac aatgacatca tccacaacga gaacatgagg caaggccggg
     2461 acaagtacaa gacgctgcgg cagatccggc agggcaacac caagcagcgc atcgacgagt
     2521 tcgaggccct gtaagaattc tgcagtcgac ggtaccgcgg gcccgggatc caccggatct
     2581 a
//
